https://scholars.lib.ntu.edu.tw/handle/123456789/598225
標題: | Rational design of a highly porous electronic scaffold with concurrent enhancement in cell behaviors and differentiation under electrical stimulation | 作者: | Chen F.-J Hsiao Y.-S Liao I.-H Liu C.-T Wu P.-I CHE-YU LIN NAI-CHEN CHENG JIASHING YU |
關鍵字: | Biocompatibility;Cell culture;Cell engineering;Conducting polymers;Multiwalled carbon nanotubes (MWCN);Scaffolds (biology);Simulation platform;Stem cells;Tissue;3D scaffolds;Adipose derived stem cells;Conductive Polymer;Electrical stimulations;Ethylenedioxythiophenes;Human adipose;Multi-walled-carbon-nanotubes;Osteogenesis;Pheochromocytoma;Poly(styrene sulfonate);Electrophysiology;biomaterial;carbon nanotube;poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate);polystyrene derivative;thiophene derivative;transcription factor RUNX2;animal;bone development;cell culture technique;cell differentiation;chemistry;cytology;devices;electronics;electrostimulation;gene expression;genetics;human;metabolism;PC12 cell line;porosity;procedures;rat;stem cell;Animals;Biocompatible Materials;Cell Culture Techniques;Cell Differentiation;Core Binding Factor Alpha 1 Subunit;Electric Stimulation;Electronics;Gene Expression;Humans;Nanotubes, Carbon;PC12 Cells;Polystyrenes;Porosity;Rats;Stem Cells;Thiophenes | 公開日期: | 2021 | 卷: | 9 | 期: | 37 | 起(迄)頁: | 7674-7685 | 來源出版物: | Journal of Materials Chemistry B | 摘要: | Conductive polymers (CPs) have received increasing attention as promising materials for studying electrophysiological signals in cell and tissue engineering. The combination of CPs with electrical stimulation (ES) could possibly enhance neurogenesis, osteogenesis, and myogenesis. To date, research has been prioritized on capitalizing CPs as two-dimensional (2D) structures for guiding the differentiation. In contrast, relatively little is conducted on the implementation of 3D conductive scaffolds. In this research, we report the synergic assembly of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) and multi-walled carbon nanotubes (MWCNTs) as a biocompatible, electrically conductive, mechanically robust and structurally porous 3D scaffold. To showcase the bioelectronic utilization, a proof-of-concept demonstration of electrically stimulated cell culture under ES is conducted. The ES effects coupled with the 3D scaffold are promising on pheochromocytoma 12 (PC12), a neuronal cell line, and the ES effect on osteogenesis of human adipose-derived stem cells (hASC) was further studied. PC12 cultured on this PEDOT:PSS/MWCNT 3D scaffolds was induced to differentiate toward a more mature neuronal phenotype with the ES treatment. Furthermore, hASC osteogenesis could be highly promoted in this conductive scaffold with ES. Calcium deposition concentration and osteo-differentiated gene markers were significantly higher with ES. The facile assembly of 3D conductive scaffolds sheds light on both platforms for investigating the 3D microenvironment for electrophysiological simulation of cells and tissues under the ES treatment ofin vivotissue engineering. ? The Royal Society of Chemistry 2021. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85116171192&doi=10.1039%2fd1tb01260f&partnerID=40&md5=71d8bc5da775662efff522a9108bd08c https://scholars.lib.ntu.edu.tw/handle/123456789/598225 |
ISSN: | 2050750X | DOI: | 10.1039/d1tb01260f |
顯示於: | 化學工程學系 |
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