https://scholars.lib.ntu.edu.tw/handle/123456789/598302
標題: | Investigation of P1/HC?pro?mediated ABA/calcium signaling responses via gene silencing through high? and low? throughput RNA?seq approaches | 作者: | Chiu Y.-H Hung Y.-L Wang H.-P Wei W.-L Shang Q.-W Pham T.H Huang C.-K Pan Z.-J CHIEN-KANG HUANG SHIH-SHUN LIN |
關鍵字: | ABA signaling;Calcium signaling;HTP?Seq;LTP?Seq;P1/HC?ProTu;Stress response;E3 ubiquitin protein ligase RDUF1;messenger RNA;P1 HC pro;protein;protein kinase;suppressor factor;transcriptome;unclassified drug;abiotic stress;abscisic acid signaling;ago1 27;Arabidopsis thaliana;arabidopsis thaliana ABA related RING type E3 ligase;arabidopsis toxicos en levadura 27;Article;biotic stress;calcium signaling;cytochrome P450 family 707 subfamily A polypeptide 3;differential gene expression;DNA library;DTX50;electrospray;gene;gene silencing;germination;high throughput sequencing;homeostasis;immune response;liquid chromatography;mass spectrometry;MEE31;molecular genetics;mutant;nonhuman;plant gene;plant growth;plant seed;plastid movement impaired1;PUB22;PUB23;real time polymerase chain reaction;RNA extraction;RNA sequencing;sensitivity analysis;signal transduction;whole transcriptome sequencing | 公開日期: | 2021 | 卷: | 13 | 期: | 12 | 來源出版物: | Viruses | 摘要: | The P1/HC?Pro viral suppressor of potyvirus suppresses posttranscriptional gene silencing (PTGS). The fusion protein of P1/HC?Pro can be cleaved into P1 and HC?Pro through the P1 self-cleavage activity, and P1 is necessary and sufficient to enhance PTGS suppression of HC?Pro. To address the modulation of gene regulatory relationships induced by turnip mosaic virus (TuMV) P1/HC?Pro (P1/HC?ProTu), a comparative transcriptome analysis of three types of transgenic plants (P1Tu, HC?ProTu, and P1/HC?ProTu) were conducted using both high?throughput (HTP) and low-throughput (LTP) RNA?Seq strategies. The results showed that P1/HC?ProTu disturbed the endogenous abscisic acid (ABA) accumulation and genes in the signaling pathway. Additionally, the inte-grated responses of stress?related genes, in particular to drought stress, cold stress, senescence, and stomatal dynamics, altered the expressions by the ABA/calcium signaling. Crosstalk among the ABA, jasmonic acid, and salicylic acid pathways might simultaneously modulate the stress responses triggered by P1/HC?ProTu . Furthermore, the LTP network analysis revealed crucial genes in common with those identified by the HTP network in this study, demonstrating the effectiveness of the miniaturization of the HTP profile. Overall, our findings indicate that P1/HC?ProTu?mediated suppression in RNA silencing altered the ABA/calcium signaling and a wide range of stress responses. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120078678&doi=10.3390%2fv13122349&partnerID=40&md5=9090527899afd2be48ae403c15cf17c5 https://scholars.lib.ntu.edu.tw/handle/123456789/598302 |
ISSN: | 19994915 | DOI: | 10.3390/v13122349 |
顯示於: | 工程科學及海洋工程學系 |
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