https://scholars.lib.ntu.edu.tw/handle/123456789/598491
標題: | Cytotoxicity and cell response of preosteoblast in calcium sulfate-augmented PMMA bone cement | 作者: | Chiang C.-C Hsieh M.-K Wang C.-Y Tuan W.-H Lai P.-L. WEI-HSING TUAN |
關鍵字: | Calcium sulfate;Cell attachment;Methyl methacrylate;Monomer;Surface roughness;Acrylic monomers;Atomic force microscopy;Bone;Bone cement;Cells;Cytology;Driers (materials);Esters;Liquid chromatography;Microporosity;Scanning electron microscopy;Sulfate minerals;Topography;Biological properties;Cell differentiation;Chemical and biologicals;Methyl methacrylates;Orthopedic applications;Particle reinforcement;Poly(methyl methacrylate) (PMMA);Ultra performance liquid chromatography;Calcium compounds;bone cement;calcium sulfate;monomer;poly(methyl methacrylate);actin filament;animal cell;Article;atomic force microscopy;cell adhesion;cell density;cell differentiation;cell structure;cell viability;compressive strength;confocal microscopy;controlled study;cytotoxicity;flexural strength;fluorescence intensity;materials testing;MC3T3-E1 cell line;mechanical test;mechanics;mouse;nonhuman;osteoblast;scanning electron microscopy;surface property;ultra performance liquid chromatography | 公開日期: | 2021 | 卷: | 16 | 期: | 5 | 來源出版物: | Biomedical Materials (Bristol) | 摘要: | Poly(methyl methacrylate) (PMMA) has been widely used in orthopedic applications, but bone ingrowth and toxic monomer release are drawback of this material. Particle reinforcement with osteoconductive substitute, such as calcium sulfate (CaSO4), is one of the solutions used to modify PMMA bone cement. The current study investigated the mechanical, chemical and biological properties of CaSO4-augmented bone cement. Mechanical strength was measured by a material testing machine. The concentration of methyl methacrylate (MMA) monomer from the various formulations of PMMA mixed with CaSO4 was measured by ultra-performance liquid chromatography (UPLC). CCK-8 assay and ALP assay were performed to evaluate cytotoxicity of released MMA monomer and cell differentiation. The attachment of cells to CaSO4-augmented bone cement discs was observed by confocal and scanning electron microscopy, and surface topography was also evaluated by atomic force microscopy. The results revealed that increased CaSO4 weight ratios led to compromised mechanical strength and increased MMA monomer release. Cell density and cell differentiation on CaSO4-augmented bone cement discs were decreased at CaSO4 weight ratios above 10%. In addition, the presence of micropores on the surface and surface roughness were both increased for PMMA composite discs containing higher levels of CaSO4. These results demonstrated that fewer MC3T3-E1 cells on the surface of CaSO4–PMMA composites was correlated to increased MMA monomer release, micropore number and surface roughness. In summary, the augmentation of a higher proportion of CaSO4 (>10 wt. %) to PMMA did not promote the biological properties of traditional PMMA bone cement. ? 2021 IOP Publishing Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114435798&doi=10.1088%2f1748-605X%2fac1ab5&partnerID=40&md5=a0429af7345ca460ae2c854660fece6a https://scholars.lib.ntu.edu.tw/handle/123456789/598491 |
ISSN: | 17486041 | DOI: | 10.1088/1748-605X/ac1ab5 |
顯示於: | 材料科學與工程學系 |
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