https://scholars.lib.ntu.edu.tw/handle/123456789/598985
標題: | The Synthesis of Europium-Doped Calcium Carbonate by an Eco-Method as Free Radical Generator Under Low-Intensity Ultrasonic Irradiation for Body Sculpture | 作者: | Kuan C.-Y Lin Y.-Y Yang I.-H Chen C.-Y Chi C.-Y Li C.-H Chen Z.-Y Lin L.-Z Yang C.-C Lin F.-H. Lin, Feng-Huei |
關鍵字: | body sculpture;calcium carbonate;europium;reactive oxygen species;ultrasound;Biocompatibility;Calcite;Calcium carbonate;Crystal structure;Inductively coupled plasma;Irradiation;Mass spectrometers;Noninvasive medical procedures;Oxygen;Particle size;Scanning electron microscopy;Sonochemistry;Tissue;Transmission electron microscopy;Ultrasonics;Body sculpture;Crystals structures;ECO method;Europium-doped;Free radical generator;Low intensity ultrasound;Low-intensity;Reactive oxygen species;Synthesised;Ultrasonics irradiations;Free radicals | 公開日期: | 2021 | 卷: | 9 | 來源出版物: | Frontiers in Bioengineering and Biotechnology | 摘要: | Body sculpture is a common method to remove excessive fat. The diet and exercise are the first suggestion to keep body shape; however, those are difficult to keep adherence. Ultrasound has been developed for fat ablation; however, it could only serve as the side treatment along with liposuction. In the study, a sonosensitizer of europium-doped calcium carbonate (CaCO3: Eu) would be synthesized by an eco-method and combined with low-intensity ultrasound for lipolysis. The crystal structure of CaCO3: Eu was identified by x-ray diffractometer (XRD). The morphology of CaCO3: Eu was analyzed by scanning electron microscope (SEM). The chemical composition of CaCO3: Eu was evaluated by energy-dispersed spectrophotometer (EDS) and inductively coupled plasma mass spectrometer (ICP-MS). The electronic diffraction pattern was to further check crystal structure of the synthesized individual grain by transmission electron microscope (TEM). The particle size was determined by Zeta-sizer. Water-soluble tetrazolium salt (WST-1) were used to evaluate the cell viability. Chloromethyl-2′,7′-dichlorofluorescein diacetate (CM-H2DCFDA) and live/dead stain were used to evaluate feasibility in vitro. SD-rat was used to evaluate the safety and efficacy in vivo. The results showed that CaCO3: Eu had good biocompatibility and could produce reactive oxygen species (ROS) after treated with low-intensity ultrasound. After 4-weeks, the CaCO3: Eu exposed to ultrasound irradiation on SD rats could significantly decrease body weight, waistline, and subcutaneous adipose tissue. We believe that ROS from sonoluminescence, CO2-bomb and locally increasing Ca2+ level would be three major mechanisms to remove away adipo-tissue and inhibit adipogenesis. We could say that the combination of the CaCO3: Eu and low-intensity ultrasound would be a non-invasive treatment for the body sculpture. Copyright ? 2021 Kuan, Lin, Yang, Chen, Chi, Li, Chen, Lin, Yang and Lin. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120736281&doi=10.3389%2ffbioe.2021.765630&partnerID=40&md5=fe7a7dd91f57adcc119a8460d5f6613c https://scholars.lib.ntu.edu.tw/handle/123456789/598985 |
ISSN: | 22964185 | DOI: | 10.3389/fbioe.2021.765630 |
顯示於: | 醫學工程學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。