https://scholars.lib.ntu.edu.tw/handle/123456789/600379
標題: | 4D bioprintable self-healing hydrogel with shape memory and cryopreserving properties | 作者: | Wu S.-D SHAN-HUI HSU |
關鍵字: | 4D bioprinting;biodegradable polyurethane;cryopreservation;self-healing;shape memory;3D printers;Ammonia;Cell culture;Cell proliferation;Crosslinking;Molecular biology;Nanoparticles;Polyurethanes;Recovery;Self-healing materials;Shape optimization;Stability;Stem cells;4d bioprinting;Biodegradable polyurethanes;Biofabrication;Bioprinting;Cryo-preservation;Neural stem cell;Property;Self-healing;Shape recovery;Shape-memory;Hydrogels;gelatin;bioprinting;hydrogel;three dimensional printing;tissue engineering;Gelatin;Printing, Three-Dimensional;Tissue Engineering | 公開日期: | 2021 | 卷: | 13 | 期: | 4 | 來源出版物: | Biofabrication | 摘要: | Four-dimensional (4D) bioprinting is an emerging biofabrication technology that integrates time as a fourth dimension with three-dimensional (3D) bioprinting for fabricating customizable tissue-engineered implants. 4D bioprinted implants are expected to possess self-healing and shape memory properties for new application opportunities, for instance, fabrication of devices with good shape integrity for minimally invasive surgery. Herein, we developed a self-healing hydrogel composed of biodegradable polyurethane (PU) nanoparticles and photo-/thermo-responsive gelatin-based biomaterials. The self-healing property of hydrogel may be associated with the formation of reversible ionomeric interaction between the COO- group of PU nanoparticles and NH3+ group on the gelatin chains. The self-healing hydrogel demonstrated excellent 3D printability and filament resolution. The UV-crosslinked printed hydrogel showed good stackability (>80 layers), structural stability, elasticity, and tunable modulus (1-60 kPa). The shape-memorizable 4D printed constructs revealed good shape fixity (~95%) and shape recovery (~98%) through the elasticity as well as forming and collapsing of water lattice in the hydrogel. The hydrogel and the printing process supported the continuous proliferation of neural stem cells (NSCs) (~3.7-fold after 14 days). Moreover, the individually bioprinted NSCs and mesenchymal stem cells in the adjacent, self-healed filaments showed mutual migration and such interaction promoted the cell differentiation behavior. The cryopreserved (-20 ~C or-80 ~C) 4D bioprinted hydrogel after awakening and shape recovery at 37 ~C demonstrated cell proliferation similar to that of the non-cryopreserved control. This 4D bioprintable, self-healable hydrogel with shape memory and cryopreserving properties may be employed for customized biofabrication. ? 2021 IOP Publishing Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117239650&doi=10.1088%2f1758-5090%2fac2789&partnerID=40&md5=12f9496cd4e4ab8eb004b7d459bd028a https://scholars.lib.ntu.edu.tw/handle/123456789/600379 |
ISSN: | 17585082 | DOI: | 10.1088/1758-5090/ac2789 |
顯示於: | 高分子科學與工程學研究所 |
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