https://scholars.lib.ntu.edu.tw/handle/123456789/601253
標題: | Regeneration of olfactory neuroepithelium in 3-methylindole-induced anosmic rats treated with intranasal chitosan | 作者: | Li S.-T Young T.-H Huang T.-W. Young, Tai-Horng |
關鍵字: | Anosmia;Apoptosis;Chitosan;Olfactory dysfunction;Olfactory neural homeostasis;Olfactory receptor neurons;Sustentacular cells;Cell culture;Cell proliferation;Insulin;Neurons;Patient treatment;Proteins;Rats;Insulin-like growth factor;Intranasal;Life qualities;Olfactory receptor neuron;Olfactory receptors;Proliferating cells;Sustentacular cell;Cell death;beta tubulin;broxuridine;caspase 3;chitosan;cytokeratin 18;olfactory marker protein;skatole;animal cell;animal experiment;animal model;animal tissue;anosmia;Article;cell fate;cell maturation;cell proliferation;cellular distribution;controlled study;DNA fragmentation;drug effect;food-seeking behavior;male;nerve cell growth;nerve regeneration;neuroapoptosis;neuroepithelium;nonhuman;olfactory receptor neuron;priority journal;protein cleavage;protein expression;rat;rat model;Sprague Dawley rat;sustentacular cell;thickness;treatment duration;animal;cell differentiation;human;olfactory mucosa;regeneration;Animals;Cell Differentiation;Humans;Olfactory Mucosa;Olfactory Receptor Neurons;Regeneration;Skatole | 公開日期: | 2021 | 卷: | 271 | 來源出版物: | Biomaterials | 摘要: | Olfactory dysfunction significantly impairs the life quality of patients but without effective treatments to date. The previous report has demonstrated that chitosan mediates the differentiation of olfactory receptor neurons (ORNs) through insulin-like growth factors and insulin-like growth factor binding protein-2 axis in an in vitro model. However, whether chitosan can further treat olfactory dysfunction in vivo remains unexplored. This study aims to evaluate the therapeutic effect of chitosan on a 3-methylindole-induced anosmic rat model. Intraperitoneal injection of 3-methylindole is performed to induce anosmia in rats. Experimental results demonstrate that the food-finding duration after chitosan treatment gradually decrease to around 80 s, and both the olfactory neuroepithelium (ON) thickness and mature ORNs (expressing olfactory marker protein) are significantly restored. Furthermore, proliferating cells (expressing bromodeoxyuridine) are mainly co-expressed with immature ORNs (expressing βIII tubulin) below the intermediate layer of the ON in the chitosan-treated group on day 28 following 3-methylindole treatment. Conversely, proliferating cells are scattered over the ON, and co-localized with immature ORNs and sustentacular cells (expressing keratin 18) in the sham group, and even immature ORNs go into apoptosis (expressing DNA fragmentation and cleaved caspase-3), possibly causing incomplete regeneration. Consequently, chitosan regenerates the ON by regulating olfactory neural homeostasis and reducing ORN apoptosis, and serves as a potential therapeutic intervention for olfactory dysfunction in the future. ? 2021 Elsevier Ltd |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102481770&doi=10.1016%2fj.biomaterials.2021.120738&partnerID=40&md5=e1e3edbdeaaa7d88873fb79aff5932d2 https://scholars.lib.ntu.edu.tw/handle/123456789/601253 |
ISSN: | 01429612 | DOI: | 10.1016/j.biomaterials.2021.120738 |
顯示於: | 醫學工程學研究所 |
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