In Vitro Fermentation of Xylooligosaccharides Produced from Miscanthus× giganteus by Human Fecal Microbiota
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
64
Journal Issue
1
Pages
262-267
Date Issued
2016
Author(s)
Swanson K.S.
Fahey G.C.
Dien B.S.
Beloshapka A.N.
Bauer L.L.
Rausch K.D.
Tumbleson M.E.
Singh V.
Abstract
Purified xylooligosaccharides from Miscanthus × giganteus (M×G XOS) were used in an in vitro fermentation experiment inoculated with human fecal microbiota. A commercial XOS product and pectin were used as controls. Decreases in pH by 2.3, 2.4, and 2.0 units and production of short-chain fatty acids (SCFA; acetic acid, 7764.2, 6664.1, and 6387.9 μmol/g; propionic acid, 1006.7, 1089.5, and 661.5 μmol/g; and butyric acid, 955.5, 1252.9, and 917.7 μmol/g) were observed in M×G XOS, commercial XOS, and pectin medium after 12 h of fermentation, respectively. Titers of Bifidobacterium spp., Lactobacillus spp., and Escherichia coli increased when fed all three substrates as monitored by qPCR. There was no significant trend for Clostridium perfringens. During fermentation, M×G XOS was statistically equivalent in performance to the commercial XOS sample as measured by culture acidification and growth of health-promoting bacteria and resulted in the highest SCFA production among the three substrates.
Publisher
American Chemical Society
Type
journal article