https://scholars.lib.ntu.edu.tw/handle/123456789/604456
標題: | Correlation of ER, PR, and HER2 at the protein and mRNA levels in Asian patients with operable breast cancer | 作者: | Chen, Chih-Jung Chen, Ting-Hao Lei, Jason Liang, Ji-An Yang, Po-Sheng CHIUN-SHENG HUANG Hsieh, Chia-Ming Tseng, Ling-Ming Liu, Liang-Chih Cheng, Skye Hung-Chen Shih, Kuan-Hui |
關鍵字: | Asian population; HER2; estrogen; gene expression; immunohistochemistry; progesterone | 公開日期: | 2022 | 出版社: | PORTLAND PRESS LTD | 卷: | 42 | 期: | 1 | 來源出版物: | Bioscience reports | 摘要: | Breast cancer is the most common cancer and the leading cause of cancer-related deaths in women. The estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) are the important biomarkers in the prognosis of breast cancer, and their expression is used to categorize breast cancer into subtypes. We aimed to analyze the concordance among ER, PR, and HER2 expression levels and breast cancer subtyping results obtained by immunohistochemistry (IHC, for protein) and reverse transcriptase-polymerase chain reaction (RT-PCR, for mRNA) and to assess the recurrence-free survival (RFS) of the different subtypes as determined by the two methods. We compared biomarker expression by IHC and RT-PCR in 397 operable breast cancer patients and categorized all patients into luminal, HER2, and triple-negative (TN) subtypes. The concordance of biomarker expression between the two methods was 81.6% (κ = 0.4075) for ER, 87.2% (κ = 0.5647) for PR, and 79.1% (κ = 0.2767) for HER2. The κ-statistic was 0.3624 for the resulting luminal, HER2, and TN subtypes. The probability of 5-year RFS was 0.78 for the luminal subtype versus 0.77 for HER2 and 0.51 for TN, when determined by IHC (P=0.007); and 0.80, 0.71, and 0.61, respectively, when determined by the RT-PCR method (P=0.008). Based on the current evidence, subtyping by RT-PCR performs similar to conventional IHC with regard to the 5-year prognosis. The PCR method may thus provide a complementary means of subtyping when IHC results are ambiguous. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/604456 | ISSN: | 0144-8463 | DOI: | 10.1042/BSR20211706 |
顯示於: | 醫學系 |
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