Measuring responses of dicyandiamide-, 3,4-dimethylpyrazole phosphate-, and allylthiourea-induced nitrification inhibition to soil abiotic and biotic factors
Journal
International Journal of Environmental Research and Public Health
Journal Volume
18
Journal Issue
13
Date Issued
2021
Author(s)
Ansari, Andrianto
Cheng, Lien-Chieh
Lin, Chiao-Ming
Wunderlich, Rainer-Ferdinand
Cao, Thanh-Ngoc-Dan
Mukhtar, Hussnain
Abstract
Nitrification inhibitors (NIs) such as dicyandiamide (DCD), 3,4-dimethylpyrazole phosphate (DMPP), and allylthiourea (AT) are commonly used to suppress ammonia oxidization at different time scales varying from a few hours to several months. Although the responses of NIs to edaphic and temperature conditions have been studied, the influence of the aforementioned factors on their inhibitory effect remains unknown. In this study, laboratory-scale experiments were conducted to assess the short-term (24 h) influence of eight abiotic and biotic factors on the inhibitory effects of DCD, DMPP, and AT across six cropped and non-cropped soils at two temperature conditions with three covariates of soil texture. Simultaneously, the dominant contributions of ammonia-oxidizing archaea (AOA) and bacteria (AOB) to potential ammonia oxidization (PAO) were distinguished using the specific inhibitor 2 phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO). Our results revealed that AT demonstrated a considerably greater inhibitory effect (up to 94.9% for an application rate of 75 mg of NI/kg of dry soil) than DCD and DMPP. The inhibitory effect of AT was considerably affected by the relative proportions of silt, sand, and clay in the soil and total PAO. In contrast to previous studies, the inhibitory effects of all three NIs remained largely unaffected by the landcover type and temperature conditions for the incubation period of 24 h. Furthermore, the efficacy of all three tested NIs was not affected by the differential contributions of AOA and AOB to PAO. Collectively, our results suggested a limited influence of temperature on the inhibitory effects of all three NIs but a moderate dependence of AT on the soil texture and PAO. Our findings can enhance the estimation of the inhibitory effect in soil, and pure cultures targeting the AOA and AOB supported ammonia oxidization and, hence, nitrogen dynamics under NI applications. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
3,4-dimethylpyrazole phosphate
Abiotic
Allylthiourea
Dicyandiamide
Inhibitory effect
Temperature
3,4 dimethylpyrazole phosphate
allylthiourea
ammonia
chemical compound
dicyandiamide
unclassified drug
dicyandiamido
guanidine derivative
phosphate
pyrazole derivative
thiourea
abiotic factor
experimental study
inhibition
nitrification
phosphate (organic)
physiological response
temperature profile
abiotic stress
Article
land use
soil texture
temperature
microbiology
oxidation reduction reaction
soil
Archaea
Ammonia
Guanidines
Nitrification
Oxidation-Reduction
Phosphates
Pyrazoles
Soil
Soil Microbiology
Thiourea
Type
journal article