https://scholars.lib.ntu.edu.tw/handle/123456789/606004
標題: | Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae | 作者: | Huang D Chao Y.-C Lv Z Jan J.-T Yang Y.-C Hsiao P.-W Wu C.-Y Liao C.-H Wu T.-H Wang L.-C. LIH-CHIANN WANG |
關鍵字: | Avian influenza virus;Clade 2.3.4.4;Silkworm pupa;Vaccine;Virus-like particle;gamma interferon;hemagglutinin;interleukin 4;matrix protein;amino acid sequence;animal cell;animal experiment;animal model;Article;avian influenza virus;Baculoviridae;blood sampling;CD4+ T lymphocyte;CD8+ T lymphocyte;chicken;controlled study;enzyme linked immunosorbent assay;Escherichia coli;immune response;immunization;immunogenicity;insect cell;mRNA expression level;nonhuman;pupa;spleen cell;Spodoptera frugiperda;transmission electron microscopy;virus like agent;Western blotting | 公開日期: | 2021 | 卷: | 65 | 期: | 2 | 起(迄)頁: | 139-145 | 來源出版物: | Journal of Veterinary Research (Poland) | 摘要: | Introduction: Novel clade 2.3.4.4 H5 highly pathogenic avian influenza virus (HPAIV) outbreaks have occurred since early 2015 in Taiwan and impacted the island economically, like they have many countries. This research investigates the immunogenicity of two HPAIV-like particles to assess their promise as vaccine candidates. Material and Methods: The haemagglutinin (HA) gene derived from clade 2.3.4.4 H5 HPAIV and matrix protein 1 (M1) gene were cloned into the pFastBac Dual baculovirus vector. The resulting recombinant viruses were expressed in Spodoptera frugiperda moth (Sf)21 cells and silkworm pupae to generate Sf21 virus-like particles (VLP) and silkworm pupa VLP. Two-week-old specific pathogen-free chickens were immunised and their humoral and cellular immune responses were analysed. Results: The silkworm pupa VLP had higher haemagglutination competence. Both VLP types elicited haemagglutination inhibition antibodies, anti-HA antibodies, splenic interferon gamma (IFN-γ) and interleukin 4 (IL-4) mRNA expression, and CD4+/CD8+ ratio elevation. However, chickens receiving silkworm pupa VLP exhibited a significantly higher anti-HA antibody titre in ELISA after vaccination. Although Sf21 VLP recipients expressed more IFN-γ and IL-4, the increase in IFN-γ did not significantly raise the CD4+/CD8+ ratio and the increase in IL-4 did not promote anti-HA antibodies. Conclusion: Both VLP systems possess desirable immunogenicity in vivo. However, in respect of immunogenic efficacy and the production cost, pupa VLP may be the superior vaccine candidate against clade 2.3.4.4 H5 HPAIV infection. ? 2021 Sciendo. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110069330&doi=10.2478%2fjvetres-2021-0026&partnerID=40&md5=01b73f25445d7183094df16b8b7a0531 https://scholars.lib.ntu.edu.tw/handle/123456789/606004 |
ISSN: | 24507393 | DOI: | 10.2478/jvetres-2021-0026 |
顯示於: | 臨床動物醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。