https://scholars.lib.ntu.edu.tw/handle/123456789/606006
標題: | A molecular and serological survey in Taiwan to determine the true risk of babesiosis in dogs not receiving regular tick prevention | 作者: | WEN-YUAN YANG Reynolds C Mestek A Huang G.-C Lee C.-J Wang S.-L. SHANG-LIN WANG |
關鍵字: | Babesiosis;Canine;Prevalence;Risk factors;Tick;edetic acid;heat shock protein 70;horseradish peroxidase;oligonucleotide;RNA 18S;adult;anemia;animal experiment;animal model;Article;Babesia;Babesia gibsoni;blood analysis;blood cell count;blood sampling;canine babesiosis;controlled study;diagnostic test accuracy study;erythrocyte count;female;hematocrit;leukocyte count;leukopenia;male;multiplex polymerase chain reaction;nonhuman;plate ELISA;platelet count;predictive value;prevalence;prophylaxis;quality control;questionnaire;real time polymerase chain reaction;risk factor;sensitivity and specificity;serology;thrombocytopenia;tick | 公開日期: | 2022 | 卷: | 27 | 來源出版物: | Veterinary Parasitology: Regional Studies and Reports | 摘要: | Canine babesiosis is an important tick-borne disease worldwide. The prevalence varies between regions and countries; however, the incidence of tick infection is associated with the status of preventive tick control measures by the owner. To date, no studies have investigated the incidence of canine babesiosis and the condition of tick prevention in Taiwan. Therefore, the true risk of babesiosis could be underestimated in dogs that are not receiving tick prophylaxis. Samples were collected at 51 hospitals around Taiwan from 265 dogs not receiving regular tick prophylaxis. Diagnostic real-time PCR was performed, and 28 dogs (10.6%) were positive for Babesia spp., including B. gibsoni (26/28) and B. vogeli (2/28). Thirty-nine dogs (14.7%) were seropositive to B. gibsoni. Take the real-time PCR positive as the Babesia infected case, the positive and negative predictive value of serological assay were 64.1% and 98.7%, respectively. The seropositivity of B. gibsoni was significantly associated with real-time PCR positivity for Babesia spp. and vice versa (p < 0.001). The odds of seropositive representing real-time PCR positivity was 132.7 times greater than the seronegative (OR: 132.731, 95% CI 35.683–493.728). Risk factors in the population identified included: dogs with a short-haired coat; intact dogs; dogs from multi-dog households; dogs with more than 10 ticks and fleas on the skin; dogs that go outdoors more than 9 times per week; and dogs with an abnormal blood test result that included anemia, thrombocytopenia, and leukopenia. However, the dogs were not tested for other co-infections, therefore, these hematological risk factors should be carefully interpreted and confirmed by further diagnostic tests. In conclusion, when dogs present with abnormal blood test results and share the risk factors listed above, babesiosis should be seriously considered and followed up with molecular and serological testing. The serological assay used in this study can provide valuable information in diagnosing babesiosis in dogs in Taiwan. ? 2021 The Authors |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120864125&doi=10.1016%2fj.vprsr.2021.100670&partnerID=40&md5=9fda91cbcbcc6643b149048b979652f6 https://scholars.lib.ntu.edu.tw/handle/123456789/606006 |
ISSN: | 24059390 | DOI: | 10.1016/j.vprsr.2021.100670 |
顯示於: | 臨床動物醫學研究所 |
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