https://scholars.lib.ntu.edu.tw/handle/123456789/606030
標題: | Microbial Phosphorylation Product of Hesperetin by Bacillus subtilis BCRC 80517 Improves Oral Bioavailability in Rats | 作者: | Wang S.-T Chen J.-A Hsu C NAN-WEI SU |
關鍵字: | Bacillus subtilis;bioavailability;bioconversion;hesperetin;hesperidin;phosphorylation;Bacteriology;Citrus fruits;Flavonoids;Phosphatases;Phosphorylation;Physicochemical properties;Rats;Solubility;ALkaline phosphatase;Aqueous solubility;Bacillus Subtilis;Bioconversion process;Intestinal permeabilities;Oral administration;Oral bioavailabilities;Water-soluble derivatives;Biochemistry;animal;Caco-2 cell line;human;rat;Animals;Biological Availability;Caco-2 Cells;Hesperidin;Humans | 公開日期: | 2021 | 卷: | 69 | 期: | 35 | 起(迄)頁: | 10184-10193 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | The flavanoid hesperidin (Hsd) is one of the major polyphenols in citrus fruits. Hsd and its aglycone hesperetin (Hst) have a broad array of bioactivities; however, their low aqueous solubility and low intestinal permeability lead to their limited oral bioavailability. In the present study, we generated two water-soluble derivatives of Hst, namely, Hst 7-O-phosphate and Hst3′-O-phosphate, by a unique bioconversion process ofBacillus subtilisvar.nattoBCRC80517. The phosphorylated products showed superior aqueous solubility and distinct physicochemical properties compared with the original Hst. The Hst phosphate derivatives (HstPs) remained stable in simulated gastric and intestinal fluids for 240 min and could revert to the original Hst form by alkaline phosphatase treatment in Caco-2 cells, showing enhanced intestinal permeabilityin vitro. After oral administration in rats, HstPs greatly elevated plasma exposure to Hst and showed better bioavailability than did Hsd. HstPs may be a potential and efficient alternative to Hst. ? 2021 American Chemical Society |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85114596984&doi=10.1021%2facs.jafc.1c04298&partnerID=40&md5=b4065cb0b05d483f6d56758a470500ba https://scholars.lib.ntu.edu.tw/handle/123456789/606030 |
ISSN: | 00218561 | DOI: | 10.1021/acs.jafc.1c04298 |
顯示於: | 農業化學系 |
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