Construction of a structural enzyme adsorption/kinetics model to elucidate additives associated lignin–cellulase interactions in complex bioconversion system
Journal
Biotechnology and Bioengineering
Journal Volume
118
Journal Issue
10
Pages
4065-4075
Date Issued
2021
Author(s)
Abstract
Enzymatic hydrolysis is a rate-limiting process in lignocellulose biorefinery. The reaction involves complex enzyme–substrate and enzyme–lignin interactions in both liquid and solid phases, and has not been well characterized numerically. In this study, a kinetic model was developed to incorporate dynamic enzyme adsorption and product inhibition parameters into hydrolysis simulation. The enzyme adsorption coefficients obtained from Langmuir isotherm were fed dynamically into first-order kinetics for simulating the equilibrium enzyme adsorption in hydrolysis. A fractal and product inhibition kinetics was introduced and successfully applied to improve the simulation accuracy on adsorbed enzyme and glucose concentrations at different enzyme loadings, lignin contents, and in the presence of bovine serum albumin (BSA) and lysozyme. The model provided numerical proof quantifying the beneficial effects of both additives, which improved the hydrolysis rate by reducing the nonproductive adsorption of enzyme on lignin. The hydrolysis rate coefficient and fractal exponent both increased with increasing enzyme loadings, and lignin inhibition exhibited with increasing fractal exponent. Compared with BSA, the addition of lysozyme exhibited higher hydrolysis rates, which was reflected in the larger hydrolysis rate coefficients and smaller fractal exponents in the simulation. The model provides new insights to support process development, control, and optimization. ? 2021 Wiley Periodicals LLC
Subjects
biorefinery
cellulase
enzymatic hydrolysis
enzyme adsorption
kinetic model
Additives
Adsorption
Cellulose derivatives
Enzymatic hydrolysis
Fractals
Isotherms
Kinetics
Lignin
Mammals
Beneficial effects
Bovine serum albumins
First order kinetics
Glucose concentration
Lignocellulose biorefinery
Process development
Rate-limiting process
Simulation accuracy
Enzyme inhibition
bovine serum albumin
cellulose
glucose
lignin
lignocellulose
lysozyme
adsorption kinetics
Article
biotransformation
concentration (parameter)
controlled study
isotherm
mathematical model
negative feedback
simulation
Cellulose Derivatives
Enzymolysis
Type
journal article