Inhibitory Effect of Garcinol on Obesity-Exacerbated, Colitis-Mediated Colon Carcinogenesis
Journal
Molecular Nutrition and Food Research
Journal Volume
65
Journal Issue
17
Date Issued
2021
Author(s)
Abstract
Scope: Epidemiological studies show a consistent and compelling association between the risk of colorectal cancer development and obesity, but its mechanisms remain poorly understood. Evidence is mounting that colorectal cancer can be prevented by nutritional supplements, such as phytochemicals. Garcinol, a polyisoprenylated benzophenone derivative, is widely present in Garcinia plants. This study investigates the potential role of garcinol supplementation in ameliorating obesity-induced colon cancer development. Methods and Results: An animal model to investigate the effect of high-fat-diet (HFD)-induced obesity on promoting colitis-associated colon cancer (AOM (azoxymethane)/DSS (dextran sodium sulfate)-induced) is designed. The results show that HFD can promote colitis-associated colon cancer as compared to an AOM/DSS group without the intervention of obesity, and supplementing with 0.05% garcinol in the diet can significantly ameliorate obesity-promoted colon carcinogenesis. The results also reveals that the microbiota composition of each group is significantly different and clustered. The most representative genera are Alistipes, Romboutsia, and Ruminococcus. The RNA-sequencing results show that the administration of garcinol can regulate genes and improve obesity-promoting colitis-associated colon carcinogenesis. Conclusion: The study results suggest that garcinol can prevent obesity-promoted colorectal cancer, and these findings provide important niches for the future development of garcinol as functional foods or adjuvant therapeutic agents. ? 2021 Wiley-VCH GmbH
Subjects
colorectal cancer
garcinol
microbiota
obesity
RNA-sequencing
antineoplastic agent
azoxymethane
biological marker
cycline
cytokine
dextran sulfate
lipid
terpene
adverse event
animal
blood
C57BL mouse
cell proliferation
colitis
colon tumor
complication
disease model
drug effect
dysbiosis
gene expression regulation
intestine flora
lipid diet
male
metabolism
organ size
pathology
physiology
Animals
Anticarcinogenic Agents
Azoxymethane
Biomarkers
Cell Proliferation
Colitis
Colonic Neoplasms
Cytokines
Dextran Sulfate
Diet, High-Fat
Disease Models, Animal
Dysbiosis
Gastrointestinal Microbiome
Gene Expression Regulation, Neoplastic
Lipids
Male
Mice, Inbred C57BL
Obesity
Organ Size
Proliferating Cell Nuclear Antigen
Terpenes
SDGs
Type
journal article