https://scholars.lib.ntu.edu.tw/handle/123456789/606169
標題: | Ganoderma formosanum exopolysaccharides inhibit tumor growth via immunomodulation | 作者: | Kuo H.-C Liu Y.-W Lum C.-C Hsu K.-D Lin S.-P Hsieh C.-W Lin H.-W Lu T.-Y KUAN-CHEN CHENG |
關鍵字: | Cytokine;Exopolysaccharides (EPS);Ganoderma formosanum;Immunomodulation;Natural killer cells;cytokine;fungal polysaccharide;animal;body weight;cell proliferation;cell survival;drug effect;drug screening;fermentation;Ganoderma;gene expression regulation;genetics;growth, development and aging;immunology;immunomodulation;Lewis carcinoma;metabolism;mouse;natural killer cell;oral drug administration;spleen;tumor cell line;tumor volume;Administration, Oral;Animals;Body Weight;Carcinoma, Lewis Lung;Cell Line, Tumor;Cell Proliferation;Cell Survival;Cytokines;Fermentation;Fungal Polysaccharides;Gene Expression Regulation, Neoplastic;Killer Cells, Natural;Mice;Spleen;Tumor Burden;Xenograft Model Antitumor Assays | 公開日期: | 2021 | 卷: | 22 | 期: | 20 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | Ganoderma formosanum (GF) is a medicinal mushroom endemic to Taiwan. Previous research established the optimal culture conditions to produce exopolysaccharide rich in β-glucan (GF-EPS) from submerged fermentation of GF. The present study investigated the antitumor effects of GF-EPS in a Lewis lung carcinoma cell (LLC1) tumor-bearing mice model. In the preventive model, GF-EPS was orally administered to mice before LLC1 injection. In the therapeutic model, GF-EPS oral administration was initiated five days after tumor cell injection. The tumor size and body weight of the mice were recorded. After sacrifice, the lymphocyte subpopulation was analyzed using flow cytometry. Spleen tissues were used to analyze cytokine mRNA expression. The results showed that GF-EPS (80 mg/kg) effectively suppressed LLC1 tumor growth in both the preventive and therapeutic models. GF-EPS administration increased the proportion of natural killer cells in the spleen and activated gene expression of several cytokines. Our results provide evidence that GF-EPS promotes tumor inhibition through immunomodulation in tumor-bearing mice. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117172545&doi=10.3390%2fijms222011251&partnerID=40&md5=241e6f5dec09c86aff45ba0c58ee54ba https://scholars.lib.ntu.edu.tw/handle/123456789/606169 |
ISSN: | 16616596 | DOI: | 10.3390/ijms222011251 |
顯示於: | 生物科技研究所 |
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