|Title:||Investigation of neuropathology after nerve release in chronic constriction injury of rat sciatic nerve||Authors:||Chen S.-H
|Keywords:||Chronic constriction injury;Compressive neuropathy;Decompression;Nerve release;Neuroinflammation;Neuropathic pain;Rat sciatic nerve;caspase 3;CD68 antigen;complementary DNA;cytokine;interleukin 1beta;isoflurane;messenger RNA;neuropeptide;protein bcl 2;tiletamine plus zolazepam;tumor necrosis factor;vasoactive intestinal polypeptide;xylazine;animal experiment;animal model;animal tissue;Article;axon;cell proliferation;chronic constriction injury;controlled study;decompression surgery;dorsal root;gastrocnemius muscle;general anesthesia;immunohistochemistry;male;mechanical allodynia;muscle atrophy;muscle mass;muscle weakness;nerve decompression;nervous system inflammation;neuroapoptosis;neuropathic pain;neuropathology;nonhuman;peripheral neuropathy;polymerase chain reaction;protein fingerprinting;rat;remyelinization;sciatic nerve;sciatic nerve injury;spinal ganglion;upregulation;animal;crush trauma;decompression surgery;denervation;gene expression profiling;genetics;hyperalgesia;immunology;inflammation;innate immunity;innervation;ligation;muscle atrophy;neuralgia;pathology;postoperative period;sciatic neuropathy;skeletal muscle;Sprague Dawley rat;Animals;Axons;Constriction;Crush Injuries;Decompression, Surgical;Denervation;Ganglia, Spinal;Gene Expression Profiling;Hyperalgesia;Immunity, Innate;Inflammation;Male;Muscle, Skeletal;Muscular Atrophy;Neuralgia;Postoperative Period;Rats;Rats, Sprague-Dawley;Remyelination;Sciatic Neuropathy||Issue Date:||2021||Journal Volume:||22||Journal Issue:||9||Source:||International Journal of Molecular Sciences||Abstract:||
Peripheral compressive neuropathy causes significant neuropathic pain, muscle weakness and prolong neuroinflammation. Surgical decompression remains the gold standard of treatment but the outcome is suboptimal with a high recurrence rate. From mechanical compression to chemical propagation of the local inflammatory signals, little is known about the distinct neuropathologic patterns and the genetic signatures after nerve decompression. In this study, controllable mechanical constriction forces over rat sciatic nerve induces irreversible sensorimotor dysfunction with sustained local neuroinflammation, even 4 weeks after nerve release. Significant gene upregulations are found in the dorsal root ganglia, regarding inflammatory, proapoptotic and neuropathic pain signals. Genetic profiling of neuroinflammation at the local injured nerve reveals persistent upregulation of multiple genes involving oxysterol metabolism, neuronal apoptosis, and proliferation after nerve release. Further validation of the independent roles of each signal pathway will contribute to molecular therapies for compressive neuropathy in the future. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
|Appears in Collections:||生物科技研究所|
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