Multiphoton imaging of autofluorescent advanced glycation end product formation in porcine aorta
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
11648
Date Issued
2021
Author(s)
Abstract
Exposure of tissues to sugar lead to the formation of advanced glycation end products (AGEs), contributing to diabetic complications. In human physiology, the vasculature is in direct contact with blood, thus the effect of diabetes is expected to be most severe with the vasculature. In this study, we incubated excised porcine aorta in D-glucose, Dgalactose, and D-fructose solutions. Multiphoton microscopy shows that for Days 4 to 48 incubation, autofluorescence is constant along the aorta sections, suggesting that monosaccharide diffusion is rapid when compared to the rate of fluorescent AGE formation. ? 2021 SPIE. All rights reserved.
Subjects
Artery; Autofluorescence; Glycation; Multiphoton imaging; Blood vessels; Glucose; Glycosylation; Advanced glycation end products; Autofluorescence; D-galactose; Direct contact; Human physiology; Multi-photon microscopy; Multiphoton imaging; Porcine aorta; Sugar industry
SDGs
Type
conference paper