Microscopic imaging of the effects of formaldehyde fixation on tissue autofluorescence
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
11648
Date Issued
2021
Author(s)
Abstract
In this work, we investigated the effect of formaldehyde-induced autofluorescence using multiphoton and confocal microscopy. Porcine aorta, brain, kidney, and ventricle were processed, sliced, and immersed in 10% neutral buffered formalin at ambient temperature for different length of time. Our preliminary results show that the inducedautofluorescence in brain and ventricle tissue increases through time, whereas for aorta and kidney it remains nearly the same. ? 2021 SPIE. All rights reserved.
Subjects
Autofluorescence; Confocal microscopy; Multiphoton microscopy; Blood vessels; Tissue; Autofluorescence; Microscopic imaging; Multiphotons; Porcine aorta; Tissue autofluorescence; Formaldehyde
Type
conference paper