https://scholars.lib.ntu.edu.tw/handle/123456789/606868
標題: | Superresolution Imaging of Photochromic Acylhydrazone Moieties on Amyloid Nanofibrils: Implications for Photoswitchable Probes | 作者: | Chen B.P.-W He R.-Y Chien H.-M Lee C.-C Chuang H.-H Hsu C.-P CHUN-CHUNG CHAN Huang J.J.-T. |
關鍵字: | acylhydrazone;amyloid;fluorophore;live-cell imaging;peptide labeling;photochromic;superresolution;Glycoproteins;Image processing;Nitrogen compounds;Peptides;Photochromism;Acylhydrazone;Condition;Hydrazones;Labelings;Live-cell imaging;Nano-fibrils;Peptide labeling;Photochromics;Super resolution imaging;Superresolution;Optical resolving power | 公開日期: | 2021 | 來源出版物: | ACS Applied Nano Materials | 摘要: | We report the incorporation of salicylaldehyde derivatives onto the hydrazine-tagged amyloidogenic peptides by forming photoisomerizable hydrazones. These hydrazones with positive photochromism are photostable under physiological conditions and enable photoswitching without the addition of external reductants or high-power irradiation. By applying superresolution microscopy, we were able to distinguish polymorphic nanoscopic structures of the hydrazone-incorporated peptides in vitro under different buffer conditions. Moreover, the additive-free condition in our platform allows the exploration of detailed amyloid aggregate morphologies in live cells. ? |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85124120581&doi=10.1021%2facsanm.1c04004&partnerID=40&md5=37488d0ccb4b737a345d71f4e0843c3b https://scholars.lib.ntu.edu.tw/handle/123456789/606868 |
ISSN: | 25740970 | DOI: | 10.1021/acsanm.1c04004 |
顯示於: | 化學系 |
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