Superresolution Imaging of Photochromic Acylhydrazone Moieties on Amyloid Nanofibrils: Implications for Photoswitchable Probes
Journal
ACS Applied Nano Materials
Date Issued
2021
Author(s)
Abstract
We report the incorporation of salicylaldehyde derivatives onto the hydrazine-tagged amyloidogenic peptides by forming photoisomerizable hydrazones. These hydrazones with positive photochromism are photostable under physiological conditions and enable photoswitching without the addition of external reductants or high-power irradiation. By applying superresolution microscopy, we were able to distinguish polymorphic nanoscopic structures of the hydrazone-incorporated peptides in vitro under different buffer conditions. Moreover, the additive-free condition in our platform allows the exploration of detailed amyloid aggregate morphologies in live cells. ?
Subjects
acylhydrazone
amyloid
fluorophore
live-cell imaging
peptide labeling
photochromic
superresolution
Glycoproteins
Image processing
Nitrogen compounds
Peptides
Photochromism
Acylhydrazone
Condition
Hydrazones
Labelings
Live-cell imaging
Nano-fibrils
Peptide labeling
Photochromics
Super resolution imaging
Superresolution
Optical resolving power
SDGs
Type
journal article