https://scholars.lib.ntu.edu.tw/handle/123456789/607024
標題: | Rapid Detection of Virus Nucleic Acid via Isothermal Amplification on Plasmonic Enhanced Digitizing Biosensor | 作者: | Wei S.-C Chang C.-C Chuang T.-L KUNG-BIN SUNG CHII-WANN LIN |
關鍵字: | LAMP;Nanoarray;Plasmonic | 公開日期: | 2022 | 卷: | 12 | 期: | 2 | 來源出版物: | Biosensors | 摘要: | Rapid detection for infectious diseases is highly demanded in diagnosis and infection prevention. In this work, we introduced a plasmonic enhanced digitizing biosensor for the rapid detection of nucleic acids. The sensor successfully achieved the detection of loop-mediated isothermal amplification for the hepatitis virus in this work. The sensor comprised a nanodisc array and Bst polymerases conjugated on the rough surface of a nanodisc. The rough surface of the nanodisc provided plasmonic hot spots to enhance the fluorescence signal. The virus DNA was detected by conducting a modified loop-mediated isothermal amplification with fluorescence resonance energy transfer reporter conjugated primers on the sensor. The modified isothermal amplification improved the signal contrast and detection time compared to the original assay. By integrating the modified amplification assay and plasmonic enhancement sensor, we achieved rapid detection of the hepatitis virus. Nucleic acid with a concentration of 10?3 to 10?4 mg/mL was detected within a few minutes by our design. Our digitizing plasmonic nanoarray biosensor also showed 20–30 min earlier detection compared to conventional loop-mediated isothermal amplification sensors. ? 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123908642&doi=10.3390%2fbios12020075&partnerID=40&md5=afe211f1614a744c55de1b5af390a2f6 https://scholars.lib.ntu.edu.tw/handle/123456789/607024 |
ISSN: | 20796374 | DOI: | 10.3390/bios12020075 |
顯示於: | 電機工程學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。