https://scholars.lib.ntu.edu.tw/handle/123456789/607027
標題: | Nyquist-exceeding high voxel rate acquisition in mesoscopic multiphoton microscopy for full-field submicron resolution resolvability | 作者: | Borah B.J Lee J.-C Chi H.-H Hsiao Y.-T CHEN-TUNG YEN CHI-KUANG SUN |
關鍵字: | Data acquisition in bioinformatics;Materials science;Optical imaging | 公開日期: | 2021 | 出版社: | Elsevier Inc. | 卷: | 24 | 期: | 9 | 起(迄)頁: | Article number 103041 | 來源出版物: | iScience | 摘要: | The Nyquist-Shannon criterion has never been realized in a laser-scanning mesoscopic multiphoton microscope (MPM) with a large field-of-view (FOV)-resolution ratio, especially when employing a high-frequency resonant-raster-scanning. With a high optical resolution nature, a current mesoscopic-MPM either neglects the criterion and degrades the digital resolution to twice the pixel size, or reduces the FOV and/or the raster-scanning speed to avoid aliasing. We introduce a Nyquist figure-of-merit (NFOM) parameter to characterize a laser-scanning MPM in terms of its optical-resolution retrieving ability. Based on NFOM, we define the maximum aliasing-free FOV, and subsequently, a cross-over excitation wavelength, below which the FOV becomes NFOM-constrained irrespective of an optimized optical design. We validate our idea in a custom-built mesoscopic-MPM with millimeter-scale FOV yielding an ultra-high FOV-resolution ratio of >3,000, while securing up-to a 1.6 mm Nyquist-satisfied aliasing-free FOV, a ?400 nm lateral resolution, and a 70 M/s effective voxel-sampling rate, all at the same time. ? 2021 The Authors |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122745017&doi=10.1016%2fj.isci.2021.103041&partnerID=40&md5=a635a9a5d21cdec0f8eafa56c8d884a9 https://scholars.lib.ntu.edu.tw/handle/123456789/607027 |
ISSN: | 25890042 | DOI: | 10.1016/j.isci.2021.103041 |
顯示於: | 電機工程學系 |
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