https://scholars.lib.ntu.edu.tw/handle/123456789/607192
標題: | Analysis of the biochemical reaction status by real-time monitoring molecular diffusion behaviors using a transistor biosensor integrated with a microfluidic channel | 作者: | Lai Y.-H Lim J.-C Lee Y.-C JIAN-JANG HUANG |
公開日期: | 2021 | 卷: | 6 | 期: | 18 | 起(迄)頁: | 11911-11917 | 來源出版物: | ACS Omega | 摘要: | Traditional methods of monitoring biochemical reactions measure certain detectable reagents or products while assuming that the undetectable species follow the stoichiometry of the reactions. Here, based upon the metal-oxide thin-film transistor (TFT) biosensor, we develop a real-time molecular diffusion model to benchmark the concentration of the reagents and products. Using the nicotinamide adenine dinucleotide (NADH)-oxaloacetic acid with the enzyme of malate dehydrogenase as an example, mixtures of different reagent concentrations were characterized to extract the ratio of remaining concentrations between NAD+ and NADH. We can thus obtain the apparent equilibrium constant of the reaction, (8.06 ± 0.61) × 104. Because the whole analysis was conducted using a TFT sensor fabricated using a semiconductor process, our approach has the advantages of exploring biochemical reaction kinetics in a massively parallel manner. ? 2021 The Authors. Published by American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105038110&doi=10.1021%2facsomega.1c00222&partnerID=40&md5=db42cdcc334b798761ff1bfb4992ec93 https://scholars.lib.ntu.edu.tw/handle/123456789/607192 |
ISSN: | 24701343 | DOI: | 10.1021/acsomega.1c00222 |
顯示於: | 電機工程學系 |
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