https://scholars.lib.ntu.edu.tw/handle/123456789/610544
標題: | Meta-lens light-sheet fluorescence microscopy for in vivo imaging | 作者: | YUAN LUO Tseng, Ming Lun Vyas, Sunil Hsieh, Ting-Yu JUI-CHING WU Chen, Shang-Yang Peng, Hsiao-Fang Su, Vin-Cent Huang, Tzu-Ting Kuo, Hsin Yu Chu, Cheng Hung Chen, Mu Ku Chen, Jia-Wern Chen, Yu-Chun KUANG-YUH HUANG CHIEH-HSIUNG KUAN Shi, Xu Misawa, Hiroaki Tsai, Din Ping |
關鍵字: | fluorescence microscopy; light sheet microscopy; metasurface; ELEGANS; OPTICS; MODEL; COLOR | 公開日期: | 2022 | 出版社: | WALTER DE GRUYTER GMBH | 卷: | 11 | 期: | 9 | 起(迄)頁: | 1949 - 1959 | 來源出版物: | Nanophotonics | 摘要: | Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/610544 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85126028360&doi=10.1515%2fnanoph-2021-0748&partnerID=40&md5=4eb9faa215c231d1a3df60393cdc7634 |
ISSN: | 2192-8606 | DOI: | 10.1515/nanoph-2021-0748 |
顯示於: | 醫學檢驗暨生物技術學系 |
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