https://scholars.lib.ntu.edu.tw/handle/123456789/613123
標題: | Oxidative Stress-Induced Alterations of Cellular Localization and Expression of Aquaporin 1 Lead to Defected Water Transport upon Peritoneal Fibrosis | 作者: | Wei, Yu-Syuan Cheng, Hui-Ping CHING-HO WU YEN-CHEN CHANG Lin, Ruo-Wei YU-TING HSU YI-TING CHEN SHUEI-LIONG LIN SU-YI TSAI SHINN-CHIH WU PEI-SHIUE TSAI |
關鍵字: | aquaporin; cytoskeleton; oxidative stress; peritoneal dialysis; porcine | 公開日期: | 30-三月-2022 | 出版社: | MDPI | 卷: | 10 | 期: | 4 | 來源出版物: | Biomedicines | 摘要: | Being one of the renal replacement therapies, peritoneal dialysis (PD) maintains around 15% of end-stage kidney disease patients' lives; however, complications such as peritoneal fibrosis and ultrafiltration failure during long-term PD compromise its application. Previously, we established a sodium hypochlorite (NaClO)-induced peritoneal fibrosis porcine model, which helped to bridge the rodent model toward pre-clinical human peritoneal fibrosis research. In this study, the peritoneal equilibration test (PET) was established to evaluate instant functional changes in the peritoneum in the pig model. Similar to observations from long-term PD patients, increasing small solutes transport and loss of sodium sieving were observed. Mechanistic investigation from both in vivo and in vitro data suggested that disruption of cytoskeleton induced by excessive reactive oxygen species defected intracellular transport of aquaporin 1, this likely resulted in the disappearance of sodium sieving upon PET. Functional interference of aquaporin 1 on free water transport would result in PD failure in patients. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/613123 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128293596&doi=10.3390%2fbiomedicines10040810&partnerID=40&md5=9b38a2b83065a8a6a3d75d8ccd4622a6 |
ISSN: | 2227-9059 | DOI: | 10.3390/biomedicines10040810 |
顯示於: | 醫學系 |
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