Digital micro-mirror device based multi-focal confocal microscopy
Journal
Proceedings of SPIE - The International Society for Optical Engineering
Journal Volume
11925
ISBN
9781510647190
Date Issued
2021-01-01
Author(s)
Abstract
For acquiring optically sectioned images of volumetric samples from multiple depths without axial scanning, a confocal system which is able to obtain multi-plane images with a Digital Micro-Mirror Device (DMD) has been proposed. The focal points of the laser beam can be arbitrarily positioned to any multiple planes in the three dimensional object space by switching multiplexed binary hologram encoded in the DMD by Lee hologram. The radius of the spherical wavefronts generated directly determines the location of the focal points and DMD can function either as a convex or concave mirror. The optical sectioning capabilities of system are verified and which can be applicable in various biomedical imaging applications.
Subjects
Confocal microscopy | DMD | Lee hologram | Multi-focal | Optical sectioning
SDGs
Type
conference paper
