https://scholars.lib.ntu.edu.tw/handle/123456789/620165
標題: | Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis | 作者: | Chen, Michael X Su, Ho-Hsuan Shiao, Ching-Ya YU-TING CHANG MING-CHU CHANG Kao, Chih-Chin Wang, San-Yuan Shih, Hsi-Chang Tsai, I-Lin |
關鍵字: | IgG4; N-glycosylation; mass spectrometry; type 1 autoimmune pancreatitis | 公開日期: | 26-十月-2021 | 出版社: | MDPI | 卷: | 22 | 期: | 21 | 起(迄)頁: | 11527 | 來源出版物: | International journal of molecular sciences | 摘要: | Type 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD), where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human IgG3 and IgG4 Fc-glycopeptides from populations in Asia were found to be isobaric ions when using LC-MS/MS as an analytical tool. In this study, an analytical workflow that coupled affinity purification and stable isotope dilution LC-MS/MS was developed to dissect IgG4 glycosylation profiles for autoimmune pancreatitis. Comparing the IgG4 and glycosylation profiles among healthy controls, patients with pancreatic ductal adenocarcinoma (PDAC), and AIP, the IgG4 glycosylations from the AIP group were found to have more digalactosylation (compared to PDAC) and less monogalactosylation (compared to HC). In addition, higher fucosylation and sialylation profiles were also discovered for the AIP group. The workflow is efficient and selective for IgG4 glycopeptides, and can be used for clinical biosignature discovery. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/620165 | ISSN: | 16616596 | DOI: | 10.3390/ijms222111527 |
顯示於: | 醫學系 |
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