https://scholars.lib.ntu.edu.tw/handle/123456789/620679
標題: | A Two-Stepped Culture Method for Efficient Production of Trichogenic Keratinocytes | 作者: | CHIH-CHIEH CHAN Fan, Sabrina Mai-Yi Wang, Wei-Hung Mu, Yi-Fen SUNG-JAN LIN |
公開日期: | 2015 | 出版社: | Mary Ann Liebert Inc. | 卷: | 21 | 期: | 10 | 起(迄)頁: | 1070-1079 | 來源出版物: | Tissue Engineering - Part C: Methods | 摘要: | Successful hair follicle (HF) neogenesis in adult life depends on the existence of both capable dermal cells and competent epidermal keratinocytes that recapitulate embryonic organogenesis through epithelial-mesenchymal interaction. In tissue engineering, the maintenance of trichogenic potential of adult epidermal cells, while expanding them remains a challenging issue. We found that although HF outer root sheath keratinocytes could be expanded for more than 100 passages as clonogenic cells without losing the proliferative potential with a 3T3J2 fibroblast feeder layer, these keratinocytes were unable to form new HFs when combined with inductive HF dermal papilla (DP) cells. However, when these high-passage keratinocytes were cocultured with HF DP cells for 4 days in vitro, they regained the trichogenic ability to form new HFs after transplantation. We found that the short-term coculture with DP cells enhanced both Wnt/β-catenin signaling, a signaling cascade key to HF development, and upregulated the expression of HF-specific genes, including K6, K16, K17, and K75, in keratinocytes, indicating that these cells were poised toward a HF fate. Hence, efficient production of trichogenic keratinocytes can be obtained by a two-stepped procedure with initial cell expansion with a 3T3J2 fibroblast feeder followed by short-term coculture with DP cells. © Copyright 2015, Mary Ann Liebert, Inc. 2015. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942901177&doi=10.1089%2ften.tec.2015.0033&partnerID=40&md5=92dea0d980c70f8c12000f74a3d37897 https://scholars.lib.ntu.edu.tw/handle/123456789/620679 |
ISSN: | 1937-3384 | DOI: | 10.1089/ten.tec.2015.0033 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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