https://scholars.lib.ntu.edu.tw/handle/123456789/620761
標題: | Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy | 作者: | Lin, M.-G. TSUNG-LIN YANG Chiang, C.-T. Kao, H.-C. Lee, J.-N. Lo, W. SHIOU-HWA JEE Chen, Y.-F. CHEN-YUAN DONG SUNG-JAN LIN |
關鍵字: | Autofluorescence; Collagen; Elastin; Multiphoton imaging; Second-harmonic generation; Thermal damage | 公開日期: | 2006 | 卷: | 11 | 期: | 6 | 來源出版物: | Journal of Biomedical Optics | 摘要: | We attempt to characterize the degree of skin thermal damage by using multiphoton microscopy to characterize dermal thermal damage. Our results show that dermal collagen and elastic fibers display different susceptibility to thermal injury. Morphologically, dermal collagen starts to denature at 60°C while fracture and aggregation of elastic fibers do not occur until 65°C. With increasing temperatures, the structures of both elastic and collagen fibers deteriorate. While second-harmonic-generation (SHG) imaging is helpful in identifying the denaturation temperature of collagen, autofluorescence (AF) imaging can help to identify the structural alternations of tissue at higher temperatures when SHG signals have decayed. We also employ a ratiometric approach based on the AF-to-SHG index of dermis (ASID) to characterize the degree of dermal thermal damage. Use of the ASID index can bypass the difficulty in analyzing inhomogeneous dermal fibers and show that dermal collagen starts to denature at 60°C. Our results suggest that with additional developments, multiphoton microscopy has potential to be developed into an effective in vivo imaging technique to monitor and characterize dermal thermal damage. © 2006 Society of Photo-Optical Instrumentation Engineers. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847336835&doi=10.1117%2f1.2405347&partnerID=40&md5=349eb44ea80dfeb56aa1215893b28397 https://scholars.lib.ntu.edu.tw/handle/123456789/620761 |
ISSN: | 1083-3668 | DOI: | 10.1117/1.2405347 | SDG/關鍵字: | article; burn; computer assisted diagnosis; human; image enhancement; in vitro study; injury; methodology; multiphoton microscopy; pathology; skin; Burns; Humans; Image Enhancement; Image Interpretation, Computer-Assisted; Microscopy, Fluorescence, Multiphoton; Skin; Collagen; Denaturation; Fluorescence microscopy; Harmonic generation; Medical imaging; Morphology; Multiphoton processes; Skin; Thermal effects; Tissue; Collagen fibers; Dermal thermal damage; Multiphoton autofluorescence; Second-harmonic-generation microscopy; Dermatology |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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