Dietary Exposure to Antibiotic Residues Facilitates Metabolic Disorder by Altering the Gut Microbiota and Bile Acid Composition
Journal
mSystems
Journal Volume
7
Journal Issue
3
Date Issued
2022-06-28
Author(s)
Chen, Rou-An
Panyod, Suraphan
Liu, Po-Yu
Chuang, Hsiao-Li
Chen, Yi-Hsun
Lyu, Qiang
Hsu, Hsiu-Ching
Lin, Tzu-Lung
Shen, Ting-Chin David
Yang, Yu-Tang
Zou, Hsin-Bai
Huang, Huai-Syuan
Lin, Yu-En
Ho, Chi-Tang
Hsu, Cheng-Chih
Abstract
Antibiotics used as growth promoters in livestock and animal husbandry can be detected in animal-derived food. Epidemiological studies have indicated that exposure to these antibiotic residues in food may be associated with childhood obesity. Herein, the effect of exposure to a residual dose of tylosin-an antibiotic growth promoter-on host metabolism and gut microbiota was explored in vivo. Theoretical maximal daily intake (TMDI) doses of tylosin were found to facilitate high-fat-diet-induced obesity, induce insulin resistance, and perturb gut microbiota composition in mice. The obesity-related phenotypes were transferrable to germfree recipient mice, indicating that the effects of a TMDI dose of tylosin on obesity and insulin resistance occurred mainly via alteration of the gut microbiota. Tylosin TMDI exposure restricted to early life, the critical period of gut microbiota development, altered the abundance of specific bacteria related to host metabolic homeostasis later in life. Moreover, early-life exposure to tylosin TMDI doses was sufficient to modify the ratio of primary to secondary bile acids, thereby inducing lasting metabolic consequences via the downstream FGF15 signaling pathway. Altogether, these findings demonstrate that exposure to very low doses of antibiotic residues, whether continuously or in early life, could exert long-lasting effects on host metabolism by altering the gut microbiota and its metabolites. IMPORTANCE This study demonstrates that even with limited exposure in early life, a residual dose of tylosin might cause long-lasting metabolic disturbances by altering the gut microbiota and its metabolites. Our findings reveal that the gut microbiota is susceptible to previously ignored environmental factors.
Subjects
bile acid metabolism; dietary exposure; early life; food safety; gut microbiota; low-dose antibiotic; metabolic disorder; obesity
Publisher
AMER SOC MICROBIOLOGY
Type
journal article