https://scholars.lib.ntu.edu.tw/handle/123456789/624603
標題: | Deletion in the s1 region of porcine epidemic diarrhea virus reduces the virulence and influences the virus-neutralizing activity of the antibody induced | 作者: | Tsai, Kuo-Jung Deng, Ming-Chung FUN-IN WANG Tsai, Shu-Hui Chang, Chieh CHIA-YI CHANG Huang, Yu-Liang |
關鍵字: | Deletion; Neutralizing active; PEDV; Spike protein; Virulence | 公開日期: | 2020 | 卷: | 12 | 期: | 12 | 來源出版物: | Viruses | 摘要: | Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and a high rate of mortality in suckling pigs. The epidemic of PEDV that occurred after 2013 was caused by non-insertion and deletion of S gene (S-INDEL) PEDV strains. During this epidemic, a variant of the non-S-INDEL PEDV strain with a large deletion of 205 amino acids on the spike gene (5-17-V) was also found to co-exist with a non-S-INDEL PEDV without deletion (5-17-O). Herein, we describe the differences in the complete genome, distribution, virulence, and antigenicity between strain 5-17-O and variant strain 5-17-V. The deletion of 205 amino acids was primarily located in the S1O domain and was associated with milder clinical signs and lower mortality in suckling pigs than those of the 5-17-O strain. The 5-17-V strain-induced antibody did not completely cross-neutralize the 5-17-O strain. In conclusion, the deletion in the S1 region reduces the virulence of PEDV and influences the virus-neutralizing activities of the antibody it induces. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85097310678&doi=10.3390%2fv12121378&partnerID=40&md5=9ea6364772b79c30d3cdd898fb3b35c6 https://scholars.lib.ntu.edu.tw/handle/123456789/624603 |
ISSN: | 19994915 | DOI: | 10.3390/v12121378 | SDG/關鍵字: | formaldehyde; phosphate buffered saline; ribonuclease inhibitor; trypsin; virus spike protein; coronavirus spike glycoprotein; neutralizing antibody; virus antibody; animal experiment; antigenicity; Article; controlled study; diarrhea; fluorescence microscopy; gene deletion; genetic similarity; high throughput sequencing; immunofluorescence assay; mortality; nonhuman; phylogeny; pig; plaque reduction neutralization test; Porcine epidemic diarrhea virus; reverse transcription polymerase chain reaction; RNA extraction; sequence alignment; Vero cell line; virus characterization; virus load; virus neutralization; virus replication; virus shedding; virus virulence; amino acid sequence; animal; antibody production; classification; Coronavirus infection; genetics; genomics; host pathogen interaction; immunology; pharmacogenetic variant; physiology; Porcine epidemic diarrhea virus; procedures; serodiagnosis; swine disease; Taiwan; veterinary medicine; virology; virulence; virus genome; whole genome sequencing; Amino Acid Sequence; Animals; Antibodies, Neutralizing; Antibodies, Viral; Antibody Formation; Coronavirus Infections; Diarrhea; Genome, Viral; Genomics; Host-Pathogen Interactions; Neutralization Tests; Pharmacogenomic Variants; Phylogeny; Porcine epidemic diarrhea virus; Sequence Deletion; Spike Glycoprotein, Coronavirus; Swine; Swine Diseases; Taiwan; Virulence; Whole Genome Sequencing |
顯示於: | 獸醫學系 |
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