https://scholars.lib.ntu.edu.tw/handle/123456789/624604
標題: | A highly conserved epitope (Rnnqipqdf) of porcine teschovirus induced a group-specific antiserum: A bioinformatics-predicted model with pan-ptv potential | 作者: | Tsai, Tung-Hsuan CHIA-YI CHANG FUN-IN WANG |
關鍵字: | Bioinformatics; Linearly arranged epitope; Porcine teschovirus | 公開日期: | 2020 | 卷: | 12 | 期: | 11 | 來源出版物: | Viruses | 摘要: | Porcine teschovirus (PTV) is an OIE-listed pathogen with 13 known PTV serotypes. Heterologous PTV serotypes frequently co-circulate and co-infect with another swine pathogen, causing various symptoms in all age groups, thus highlighting the need for a pan-PTV diagnostic tool. Here, a recombinant protein composed of a highly conserved “RNNQIPQDF” epitope on the GH loop of VP1, predicted in silico, and a tandem repeat of this epitope carrying the pan DR (PADRE) and Toxin B epitopes was constructed to serve as a PTV detection tool. This recombinant GST-PADRE-(RNNQIPQDF)n-Toxin B protein was used as an immunogen, which effectively raised non-neutralizing or undetectable neutralizing antibodies against PTV in mice. The raised antiserum was reactive against all the PTV serotypes (PTV–1–7) tested, but not against members of the closely related genera Sapelovirus and Cardiovirus, and the unrelated virus controls. This potential pan-PTV diagnostic reagent may be used to differentiate naturally infected animals from vaccinated animals that have antibodies against a subunit vaccine that does not contain this epitope or to screen for PTV before further subtyping. To our knowledge, this is the first report that utilized in silico PTV epitope prediction to find a reagent broadly reactive to various PTV serotypes. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85095389271&doi=10.3390%2fv12111225&partnerID=40&md5=5a762ce0730883711f256aa057b26064 https://scholars.lib.ntu.edu.tw/handle/123456789/624604 |
ISSN: | 19994915 | DOI: | 10.3390/v12111225 | SDG/關鍵字: | antiserum; epitope; Freund adjuvant; fusion protein; glutathione transferase; plasmid vector; protein VP1; epitope; neutralizing antibody; recombinant protein; amino acid sequence; animal cell; animal experiment; animal model; antibody detection; antibody response; Article; bioinformatics; Cardiovirus; cell line; computer model; conserved sequence; controlled study; cytopathogenic effect; DNA sequencing; Encephalomyocarditis virus; enzyme activity; female; hydrophilicity; immunization; immunoblotting; immunofluorescence assay; immunogenicity; immunohistochemistry; indirect ELISA; molecular cloning; molecular epidemiology; mouse; nonhuman; polyacrylamide gel electrophoresis; polymerase chain reaction; protein expression; RNA binding; Sapelovirus; sequence alignment; serotype; SK-RST cell line; Teschovirus; virus inactivation; virus load; virus neutralization; Western blotting; animal; biology; computer simulation; epitope mapping; genetics; immunology; pathogenicity; pig; procedures; swine disease; Teschovirus; virology; Animals; Antibodies, Neutralizing; Computational Biology; Computer Simulation; Epitope Mapping; Epitopes; Female; Immune Sera; Mice; Recombinant Proteins; Serogroup; Swine; Swine Diseases; Teschovirus |
顯示於: | 獸醫學系 |
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