https://scholars.lib.ntu.edu.tw/handle/123456789/624626
標題: | Isolation and characterization of a Sagiyama virus from domestic pigs | 作者: | CHIA-YI CHANG Huang, Chin-Cheng Huang, Tien-Shine Deng, Ming-Chung Jong, Ming-Hwa FUN-IN WANG |
關鍵字: | Getah virus; Pigs; Ross River virus; Sagiyama virus | 公開日期: | 2006 | 卷: | 18 | 期: | 2 | 起(迄)頁: | 156-161 | 來源出版物: | Journal of Veterinary Diagnostic Investigation | 摘要: | In 2002, a strain of Sagiyama virus (SAGV) designated ML/Taiwan/02 was isolated from farmed pigs in Taiwan. The nsP1 and E1 gene sequences of the ML/Taiwan/02 strain shared 98.6 and 96.7% homology, respectively, with corresponding genes of a Japanese strain of SAGV. Nucleotide and amino acid sequence comparison revealed this strain of SAGV to be most closely related to Getah virus, as opposed to its current classification as a subtype of Ross River virus. To investigate the seroprevalence of SAGV infection in Taiwan, a total of 586 pig sera collected from 11 of 17 Taiwanese districts were tested for serum neutralizing antibodies (SNA) against SAGV. Results indicated that 51% of the samples had SNA titer ≥4, and 40% had SNA titer ≥48, indicative of repeated exposure to SAGV in the field. To study the pathogenicity of the ML/ Taiwan/02 strain, this strain was experimentally inoculated into 4-week-old specific-pathogen-free pigs that were seronegative for SAGV. Viremia was detected during postinoculation days (PID) 2-4, when the SNA titer was ≤16. By PID 7, viremia was no longer detectable, coinciding with the increase of SNA titer to ≥48. Clinical illnesses or remarkable lesions were not observed. To the authors' knowledge, this is the first reported isolation of a strain of SAGV from pigs in the field. The virus is experimentally nonpathogenic to pigs but is moderately widespread, most likely via repeated exposure to virus-carrying mosquitoes. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645338158&doi=10.1177%2f104063870601800203&partnerID=40&md5=726e1770949163ede724f502f80d4fc3 https://scholars.lib.ntu.edu.tw/handle/123456789/624626 |
ISSN: | 10406387 | DOI: | 10.1177/104063870601800203 | SDG/關鍵字: | Getah virus; Ross River virus; Sagiyama virus; Sus scrofa; Sus scrofa domestica |
顯示於: | 獸醫學系 |
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