https://scholars.lib.ntu.edu.tw/handle/123456789/624840
標題: | N6-methyladenosine modification of lncRNA Pvt1 governs epidermal stemness | 作者: | Lee J Wu Y Harada B.T Li Y Zhao J He C Ma Y Wu X. LI-HSING LEE |
關鍵字: | epidermal progenitor cell; lncRNA; m6A modification; Pvt1 signaling | 公開日期: | 2021 | 卷: | 40 | 期: | 8 | 來源出版物: | EMBO Journal | 摘要: | Dynamic chemical modifications of RNA represent novel and fundamental mechanisms that regulate stemness and tissue homeostasis. Rejuvenation and wound repair of mammalian skin are sustained by epidermal progenitor cells, which are localized within the basal layer of the skin epidermis. N6-methyladenosine (m6A) is one of the most abundant modifications found in eukaryotic mRNA and lncRNA (long noncoding RNA). In this report, we survey changes of m6A RNA methylomes upon epidermal differentiation and identify Pvt1, a lncRNA whose m6A modification is critically involved in sustaining stemness of epidermal progenitor cells. With genome-editing and a mouse genetics approach, we show that ablation of m6A methyltransferase or Pvt1 impairs the self-renewal and wound healing capability of skin. Mechanistically, methylation of Pvt1 transcripts enhances its interaction with MYC and stabilizes the MYC protein in epidermal progenitor cells. Our study presents a global view of epitranscriptomic dynamics that occur during epidermal differentiation and identifies the m6A modification of Pvt1 as a key signaling event involved in skin tissue homeostasis and wound repair. © 2021 The Authors |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102593273&doi=10.15252%2fembj.2020106276&partnerID=40&md5=66d1ed8e43340995679ebe1b7da5c237 https://scholars.lib.ntu.edu.tw/handle/123456789/624840 |
ISSN: | 02614189 | DOI: | 10.15252/embj.2020106276 | SDG/關鍵字: | 6 n methyladenosine; long untranslated RNA; Myc protein; adenosine; long untranslated RNA; methyltransferase; Mettl14 protein, mouse; Myc protein; N-methyladenosine; protein binding; PVT1 long-non-coding RNA, mouse; adult; animal cell; animal experiment; animal tissue; Article; cell differentiation; cell self-renewal; chemical modification; controlled study; epidermis cell; gene; gene editing; gene identification; genetic analysis; homeostasis; human; human tissue; male; Mettl14 gene; mouse; nonhuman; priority journal; protein protein interaction; Pvt1 gene; signal transduction; stem cell; transcriptomics; wound healing; animal; cell culture; cell differentiation; cytology; epidermis cell; genetics; guinea pig; metabolism; physiology; RNA processing; Adenosine; Animals; Cell Differentiation; Cells, Cultured; Epidermal Cells; Guinea Pigs; Methyltransferases; Mice; Protein Binding; Proto-Oncogene Proteins c-myc; RNA Processing, Post-Transcriptional; RNA, Long Noncoding; Stem Cells; Wound Healing |
顯示於: | 病理學科所 |
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