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  4. Structural basis for promiscuity and specificity during Candida glabrata invasion of host epithelia
 
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Structural basis for promiscuity and specificity during Candida glabrata invasion of host epithelia

Journal
Proceedings of the National Academy of Sciences of the United States of America
Journal Volume
109
Journal Issue
42
Pages
16864-16869
Date Issued
2012
Author(s)
MANUEL MAESTRE-REYNA  
Diderrich R
Veelders M.S
Eulenburg G
Kalugin V
Bruc̈kner S
Keller P
Rupp S
Mos̈ch H.-U
Essen L.-O.
DOI
10.1073/pnas.1207653109
URI
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867646292&doi=10.1073%2fpnas.1207653109&partnerID=40&md5=a036de5ba6801f039559ee0c4774b376
https://scholars.lib.ntu.edu.tw/handle/123456789/624884
Abstract
The human pathogenic yeast Candida glabrata harbors more than 20 surface-exposed, epithelial adhesins (Epas) for host cell adhesion. The Epa family recognizes host glycans and discriminates between target tissues by their adhesin (A) domains, but a detailed structural basis for ligand-binding specificity of Epa proteins has been lacking so far. In this study, we provide high-resolution crystal structures of the Epa1A domain in complex with different carbohydrate ligands that reveal how host cell mucin-type O-glycans are recognized and allow a structure-guided classification of the Epa family into specific subtypes. Further detailed structural and functional characterization of subtype-switched Epa1 variants shows that specificity is governed by two inner loops, CBL1 and CBL2, involved in calcium binding as well as by three outer loops, L1, L2, and L3. In summary, our study provides the structural basis for promiscuity and specificity of Epa adhesins, which might further contribute to developing anti-adhesive antimycotics and combating Candida colonization.
Subjects
Fungal pathogen; Lectin; Molecular recognition; T-antigen
Other Subjects
carbohydrate; Epa1A protein; fungal protein; glycan; mucin; unclassified drug; article; calcium binding; Candida glabrata; candidiasis; crystal structure; crystallization; epithelium; fungal colonization; host; nonhuman; phylogeny; priority journal; protein analysis; protein domain; protein structure; Calcium; Candida glabrata; Cluster Analysis; Computational Biology; Crystallography, X-Ray; Fluorescence; Fungal Proteins; Lectins; Models, Molecular; Multigene Family; Phylogeny; Polysaccharides; Protein Binding; Protein Conformation
Type
journal article

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