https://scholars.lib.ntu.edu.tw/handle/123456789/625280
標題: | Fabrication of in situ magnetic capturing and Raman enhancing nanoplatelets for detection of bacteria and biomolecules | 作者: | Juang R.-S Chen W.-T Cheng Y.-W Wang K.-S RU-JONG JENG Zeng Z.-L Liu S.-H Liu T.-Y. |
關鍵字: | Au nanoparticles; Magnetic capturing; Magnetic nanoparticles; Nanoscale silicate platelets; SERS detection | 公開日期: | 2022 | 卷: | 648 | 來源出版物: | Colloids and Surfaces A: Physicochemical and Engineering Aspects | 摘要: | In situ magnetic capturing and surface-enhanced Raman scattering (SERS) detection techniques can be used for detecting microbial contamination and uremic toxins. Magnetic SERS substrates were fabricated using immobilized gold nanoparticles (AuNPs) and iron-oxide (Fe3O4) nanoparticles on exfoliated nanoscale silicate platelets (NSPs). The prepared magnetic SERS nanosheets (Fe3O4@AuNPs@NSP nanosheets) were able to not only magnetically capture bacteria and biomolecules but also effectively induce the hot-spot effect and enhance the Raman signal by the surface plasmon resonance of the AuNP arrays. The results showed that both the ratio of AuNPs to Fe3O4 nanoparticles and the order of immobilization on the NSP nanosheets are important factors for inducing magnetic capturing behavior and SERS sensitivity. A method that yielded magnetic capturing behavior was to graft AuNPs on NSP nanosheets first, followed by the immobilization of Fe3O4 nanoparticles. Fe3O4@AuNPs@NSP nanosheets prepared using 0.9 mM HAuCl4 displayed the highest magnetic capturing behavior and SERS enhancement effect, showing an optimal interparticle gap. The bacteria (Escherichia coli) were captured and separated by the magnetic SERS substrates using an applied magnetic field, and then the magnetically captured samples were monitored by Raman spectroscopy for rapid SERS detection. Furthermore, the SERS sensitivity increased by ~2 times after magnetic capturing, and the limit of detection for sensing bacteria was below 103 CFU/mL. The novel magnetic SERS substrates provide ultrasensitive, rapid, and reproducible SERS detection for magnetically capturing biomolecules (bacteria, uremic toxins, and parathyroid hormone) and can be applied in environmental, water, and biomedical detection. © 2022 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130324166&doi=10.1016%2fj.colsurfa.2022.129189&partnerID=40&md5=871b7781894d2ef082c7c3c728ea6cd2 https://scholars.lib.ntu.edu.tw/handle/123456789/625280 |
ISSN: | 09277757 | DOI: | 10.1016/j.colsurfa.2022.129189 | SDG/關鍵字: | Biomolecules; Chlorine compounds; Escherichia coli; Fabrication; Gold compounds; Gold nanoparticles; Nanomagnetics; Nanosheets; Raman scattering; Raman spectroscopy; Silicates; Substrates; Surface plasmon resonance; Surface scattering; Au nanoparticle; Enhanced Raman scattering; Magnetic capturing; Magnetic surfaces; Nano scale; Nanoscale silicate platelet; Raman scattering substrate; Silicate platelets; Surface enhanced Raman; Surface-enhanced raman scattering detection; Magnetite; adenine; adenosine phosphate; gold nanoparticle; iron oxide nanoparticle; nanoplatelet; nanosheet; polyamine; uric acid; aqueous solution; Article; bacterium detection; chemical composition; colony forming unit; comparative study; controlled study; Escherichia coli; immobilization; limit of detection; magnetic field; nonhuman; optical spectroscopy; Raman spectrometry; scanning electron microscopy; surface area; surface plasmon resonance; surface property; ultraviolet radiation |
顯示於: | 高分子科學與工程學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。