Intelligent and thermo-responsive Au-pluronic® F127 nanocapsules for Raman-enhancing detection of biomolecules
Journal
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Journal Volume
279
Date Issued
2022
Author(s)
Abstract
Thermo-responsive Raman-enhanced nanocapsules were successfully fabricated by Pluronic® F127 (F127) decorated with gold nanoparticles (AuNPs) for surface-enhanced Raman scattering (SERS) detection of biomolecules. F127 nanocapsules changes from hydrophilicity (swelling) to hydrophobicity (de-swelling) when the temperature increases from 15 °C to 37 °C, owing to the lower critical solution temperature (LCST) of F127 is about 26.5 °C. The size of nanocapsules would be enormous shrinking from 160 nm to 20 nm, resulting in a significant decrease in the distance between AuNPs to enhance hot spot effect, which increases the sensitivity of SERS detection. Based on the thermo-sensitive behavior, the ratio of AuNPs and F127 would be manipulated to find the optimal SERS enhancement effect. SERS nanocapsules can rapidly detect biomolecules (adenine and R6G) with limit of detection (LOD) lower than 10−6 M. In addition, the relatively difficult to detect clinical samples, carboxyl-terminal parathyroid hormone fragments (C-PTH), can also be measured by the thermo-responsive SERS nanocapsules developed in this work. It is expected the biomolecules can be adsorbed at low temperature (15 °C), as well as collected and concentrated at high temperature (37 °C) for SERS detection, to increase the sensitivity and stability of SERS detection. © 2022 Elsevier B.V.
Subjects
Bio-detection; Gold nanoparticles; Pluronic F127; surface-enhanced Raman scattering (SERS) detection; Thermo-responsive polymer
Other Subjects
Biomolecules; Fiber optic sensors; Metal nanoparticles; Nanocapsules; Raman scattering; Surface scattering; Temperature; Biodetection; Enhanced Raman scattering; Gold nanoparticle; Gold Nanoparticles; Pluronic F-127; Pluronics; Surface enhanced Raman; Surface-enhanced raman scattering detection; Thermo-responsive; Thermoresponsive polymer; Gold nanoparticles; gold; metal nanoparticle; nanocapsule; poloxamer; polyethylene derivative; polypropylene; UCON 50-HB-5100; procedures; Raman spectrometry; Gold; Metal Nanoparticles; Nanocapsules; Poloxamer; Polyethylenes; Polypropylenes; Spectrum Analysis, Raman
Type
journal article