Towards an Electrochemical Immunosensor for the Detection of Antibodies against SARS-CoV-2 Spike Protein
Journal
Journal of the Electrochemical Society
Journal Volume
169
Journal Issue
3
Date Issued
2022
Author(s)
Liustrovaite V
Drobysh M
Rucinskiene A
Baradoke A
Ramanaviciene A
Plikusiene I
Samukaite-Bubniene U
Viter R
Ramanavicius A.
Abstract
Herein we report the electrochemical system for the detection of specific antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins in blood serum patient samples after coronavirus disease 2019 (COVID-19). For this purpose, the recombinant SARS-CoV-2 spike protein (SCoV2-rS) was covalently immobilised on the surface of the gold electrode pre-modified with mixed self-assembled monolayer (SAMmix) consisting of 11-mercaptoundecanoic acid and 6-mercapto-1-hexanol. The affinity interaction of SCoV2-rS with specific antibodies against this protein (anti-rS) was detected using two electrochemical methods: cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The anti-rS was detected with a detection limit of 2.53 nM and 1.99 nM using CV and EIS methods, respectively. The developed electrochemical immunosensor is suitable for the confirmation of COVID-19 infection or immune response in humans after vaccination. © 2022 The Author(s
Subjects
Antigen-antibody complex.; COVID-19; Cyclic voltammetry; Electrochemical immunosensor; Electrochemical impedance spectroscopy; SARS-CoV-2; Self-assembled monolayer (SAM)
SDGs
Other Subjects
Antibodies; Chemical detection; Cyclic voltammetry; Electrochemical impedance spectroscopy; Immunosensors; Recombinant proteins; Self assembled monolayers; Antigen-antibody complex; Antigen-antibody complex.; Coronavirus disease 2019; Coronaviruses; Electrochemical immunosensors; Electrochemical-impedance spectroscopies; Self-assembled monolayer; Severe acute respiratory syndrome coronavirus; Severe acute respiratory syndrome coronavirus 2; Spike protein; Coronavirus
Type
journal article