https://scholars.lib.ntu.edu.tw/handle/123456789/625652
標題: | Chitosan augments photodynamic inactivation of gram-positive and gram-negative bacteria | 作者: | Tsai T Chien H.-F Wang T.-H CHING-TSAN HUANG Ker Y.-B CHIN-TIN CHEN |
公開日期: | 2011 | 卷: | 55 | 期: | 5 | 起(迄)頁: | 1883-1890 | 來源出版物: | Antimicrobial Agents and Chemotherapy | 摘要: | Antimicrobial photodynamic inactivation (PDI) was shown to be a promising treatment modality for microbial infections. This study explores the effect of chitosan, a polycationic biopolymer, in increasing the PDI efficacy against Gram-positive bacteria, including Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, and methicillin-resistant S. aureus (MRSA), as well as the Gram-negative bacteria Pseudomonas aeruginosa and Acinetobacter baumannii. Chitosan at <0.1% was included in the antibacterial process either by coincubation with hematoporphyrin (Hp) and subjection to light exposure to induce the PDI effect or by addition after PDI and further incubation for 30 min. Under conditions in which Hp-PDI killed the microbe on a 2- to 4-log scale, treatment with chitosan at concentrations of as low as 0.025% for a further 30 min completely eradicated the bacteria (which were originally at ∼10 8 CFU/ml). Similar results were also found with toluidine blue O (TBO)-mediated PDI in planktonic and biofilm cells. However, without PDI treatment, chitosan alone did not exert significant antimicrobial activity with 30 min of incubation, suggesting that the potentiated effect of chitosan worked after the bacterial damage induced by PDI. Further studies indicated that the potentiated PDI effect of chitosan was related to the level of PDI damage and the deacetylation level of the chitosan. These results indicate that the combination of PDI and chitosan is quite promising for eradicating microbial infections. Copyright © 2011, American Society for Microbiology. All Rights Reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955535255&doi=10.1128%2fAAC.00550-10&partnerID=40&md5=0fad35f4bfd00753b390909aabcfc590 https://scholars.lib.ntu.edu.tw/handle/123456789/625652 |
ISSN: | 00664804 | DOI: | 10.1128/AAC.00550-10 | SDG/關鍵字: | chitosan; hematoporphyrin; tolonium chloride; Acinetobacter baumannii; antibacterial activity; antimicrobial activity; article; bacterial cell; biofilm; cell survival; colony forming unit; concentration (parameters); controlled study; deacetylation; Gram negative bacterium; Gram positive bacterium; incubation time; light exposure; methicillin resistant Staphylococcus aureus; molecular weight; nonhuman; photodynamic inactivation; photodynamics; priority journal; Pseudomonas aeruginosa; Staphylococcus aureus; Staphylococcus epidermidis; Streptococcus pyogenes; Acinetobacter baumannii; Anti-Bacterial Agents; Biofilms; Chitosan; Gram-Negative Bacteria; Gram-Positive Bacteria; Hematoporphyrins; Microbial Sensitivity Tests; Polylysine; Pseudomonas aeruginosa; Staphylococcus aureus; Staphylococcus epidermidis |
顯示於: | 生化科技學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。