https://scholars.lib.ntu.edu.tw/handle/123456789/625671
標題: | Functional characterization of the human placental fusogenic membrane protein syncytin 2 | 作者: | Chen C.-P Chen L.-F Yang S.-R Chen C.-Y Ko C.-C GEEN-DONG CHANG Chen H. |
關鍵字: | Cell fusion; Placenta; Pregnancy; Syncytin 1; Syncytin 2; Syncytiotrophoblast; Trophoblast | 公開日期: | 2008 | 卷: | 79 | 期: | 5 | 起(迄)頁: | 815-823 | 來源出版物: | Biology of Reproduction | 摘要: | Fusion of cytotrophoblasts into the multinucleated syncytiotrophoblast layer is essential for the development of a functional placenta. The envelope protein of a human endogenous retrovirus W (HERV-W) family member, syncytin 1, has been shown to mediate placental cell fusion. Recently, the envelope protein of another HERV family member (HERV-FRD), syncytin 2, has been identified and shown to be highly expressed in the placenta. To better understand the biology of syncytin 2, in this study we first investigated syncytin 2 gene expression in normal and preeclamptic placentas and then characterized the functions of syncytin 2. The expression of syncytin 2 gene was decreased in preeclamptic placentas and could be stimulated by the cAMP stimulant forskolin. The endoprotease furin was found to be involved in the posttranslational cleavage of syncytin 1 and 2 polypeptides into surface and transmembrane subunits. In addition, proper association of the subunits of syncytins 1 and 2 is probably required for the functional integrity of each protein, because subunit swapping of syncytins 1 and 2 failed to generate fusogenic chimeras. Finally, we demonstrated that the disulfide bridge-forming CX2C and CX 7C motifs found in syncytins 1 and 2 are essential for their fusogenic activities, because mutations in the CX2C motif not only abolished fusogenesis but also functioned as dominant-negative mutants. Our results suggest that syncytin 2 may function as a second fusogenic protein for placental cell fusion. © 2008 by the Society for the Study of Reproduction, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-55549115075&doi=10.1095%2fbiolreprod.108.069765&partnerID=40&md5=505db3e0b92bc507736fbbf70383c0e2 https://scholars.lib.ntu.edu.tw/handle/123456789/625671 |
ISSN: | 00063363 | DOI: | 10.1095/biolreprod.108.069765 | SDG/關鍵字: | envelope protein; forskolin; furin; membrane protein; syncytin 2 protein; unclassified drug; article; cell fusion; cytotrophoblast; gene expression; genetic transfection; human; human cell; human tissue; immunoblotting; immunohistochemistry; immunoprecipitation; placenta development; preeclampsia; priority journal; protein expression; protein function; protein processing; real time polymerase chain reaction; RNA isolation; syncytiotrophoblast; Case-Control Studies; Cell Fusion; Cell Line; Enzyme Activators; Female; Forskolin; Furin; Gene Expression Regulation; Gene Products, env; Humans; Pre-Eclampsia; Pregnancy; Pregnancy Proteins; Protein Structure, Tertiary; Structure-Activity Relationship; Human endogenous retrovirus W |
顯示於: | 生化科學研究所 |
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