https://scholars.lib.ntu.edu.tw/handle/123456789/625700
標題: | WHIRLY1 recruits the histone deacetylase HDA15 repressing leaf senescence and flowering in Arabidopsis | 作者: | Huang D Lan W Ma W Huang R Lin W Li M Chen C.-Y KEQIANG WU Miao Y. |
關鍵字: | flowering; H3K9 acetylation; HDA15; leaf senescence; multi-omics analysis; WHY1 | 公開日期: | 2022 | 來源出版物: | Journal of Integrative Plant Biology | 摘要: | Leaf senescence is controlled by a complex regulatory network in which robustness is ensured by the activity of transcription factors and epigenetic regulators. However, how these coordinate the process of leaf senescence remains poorly understood. We found that WHIRLY1 interacts with Histone Deacetylase (HDA)15, a Reduced Potassium Dependence3 (RPD3)/HDA1-type HDA, by using green fluorescent protein-nanotrap-mass spectrum assays. The development-dependent interaction between WHIRLY1 and HDA15 was further confirmed by bimolecular fluorescence complementation assays and co-immunoprecipitation assays in Arabidopsis. Multi-omics genome-wide transcriptome and H3K9 acetylome enrichment analysis showed that HDA15 delays leaf senescence and flowering by repressing the expression of the positive regulators of leaf senescence and flowering, such as LOX2 and LARP1C, and reducing H3K9ac levels at these loci; WHIRLY1 and HDA15 co-target to the region near the transcription start site of a subset of nutrient recycling-related genes (e.g., Glutathione S-transferases 10, non-coding RNA, and photosystem II protein D1 synthesizer attenuator PDIL1-2), as well as WRKY53 and ELF4, and co-repress their expression by removing H3K9 acetylation. Our study revealed a key transcription regulatory node of nutrient recycling and senescence-associated genes involved in leaf senescence and flowering via the recruitment of HDA15 by the single-stranded DNA/RNA-binding protein WHIRLY1. © 2022 The Authors. Journal of Integrative Plant Biology published by John Wiley & Sons Australia, Ltd on behalf of Institute of Botany, Chinese Academy of Sciences. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131805236&doi=10.1111%2fjipb.13272&partnerID=40&md5=f209bbaaadc635918a3c06403e04bc6c https://scholars.lib.ntu.edu.tw/handle/123456789/625700 |
ISSN: | 16729072 | DOI: | 10.1111/jipb.13272 |
顯示於: | 植物科學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。