https://scholars.lib.ntu.edu.tw/handle/123456789/625906
標題: | Impact of Storage Condition on Chemical Composition and Antifungal Activity of Pomelo Extract against Colletotrichum gloeosporioides and Anthracnose in Post-harvest Mango | 作者: | Cheng, Yu Jung Wu, Ying Jou Lee, Fang Wei Ou, Ling Yi Chen, Chi Nan Chu, Yu Ying YEN-CHOU KUAN |
關鍵字: | anthracnose | antifungal | edible coating | mango | pomelo peel | post-harvest disease | 公開日期: | 八月-2022 | 出版社: | MDPI | 卷: | 11 | 期: | 15 | 來源出版物: | Plants | 摘要: | Anthracnose caused by Colletotrichum leads to a tremendous post-harvest mango loss. While chemical fungicides are applied to control anthracnose, natural alternatives are preferred due to food safety and environmental concerns. Pomelo extract (PE) exhibits a broad spectrum of antimicrobial activities; however, its effect against anthracnose is unknown. Here we investigated the chemical profile of PE using GC-MS and the anti-anthracnose activity of PE using in vitro and in vivo assays. We also evaluated the impact of storage temperature (0°, 5°, 10°, 20°, −20°, and −80 °C) and light conditions on the composition and antifungal activity of PE. We found that PE inhibited C. gloeosporioides in vitro with an IC50 of 3.2 mL L−1. Applying chitosan-based coating incorporated with 20 mL L−1 PE significantly suppressed anthracnose in post-harvest ‘Keitt’ mango. A storage temperature below 5 °C substantially preserved major compounds and the antifungal activity of PE after 6 m of storage. Finally, we showed that applying d-limonene, the key constituent of PE, inhibited C. gloeosporioides in vitro (IC50: 10.9 mM) and suppressed anthracnose in vivo. In conclusion, we demonstrated that the application of PE and d-limonene are sustainable methods for anthracnose control in post-harvest crops and established the preservation protocol for PE. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/625906 | ISSN: | 2223-7747 | DOI: | 10.3390/plants11152064 |
顯示於: | 園藝暨景觀學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。