https://scholars.lib.ntu.edu.tw/handle/123456789/626020
標題: | CFM6 is an Essential CRM Protein Required for the Splicing of nad5 Transcript in Arabidopsis Mitochondria | 作者: | Lin, Wei Chih Chen, Ya Huei Gu, Shin Yuan Shen, Hwei Ling Huang, Kai Chau Lin, Wen Dar MEN-CHI CHANG ING-FENG CHANG CHWAN-YANG HONG Cheng, Wan Hsing |
關鍵字: | Arabidopsis thaliana; CRM domain-containing protein; Embryogenesis; Group II intron; Male sterility; Mitochondrial respiratory complex I | 公開日期: | 2022 | 卷: | 63 | 期: | 2 | 起(迄)頁: | 217-233 | 來源出版物: | Plant and Cell Physiology | 摘要: | Plant chloroplast RNA splicing and ribosome maturation (CRM)-domain-containing proteins are capable of binding RNA to facilitate the splicing of group I or II introns in chloroplasts, but their functions in mitochondria are less clear. In the present study, Arabidopsis thaliana CFM6, a protein with a single CRM domain, was expressed in most plant tissues, particularly in flower tissues, and restricted to mitochondria. Mutation of CFM6 causes severe growth defects, including stunted growth, curled leaves, delayed embryogenesis and pollen development. CFM6 functions specifically in the splicing of group II intron 4 of nad5, which encodes a subunit of mitochondrial complex I, as evidenced by the loss of nad5 intron 4 splicing and high accumulation of its pretranscripts in cfm6 mutants. The phenotypic and splicing defects of cfm6 were rescued in transgenic plants overexpressing 35S:CFM6-YFP. Splicing failure in cfm6 also led to the loss of complex I activity and to its improper assembly. Moreover, dysfunction of complex I induced the expression of proteins or genes involved in alternative respiratory pathways in cfm6. Collectively, CFM6, a previously uncharacterized CRM domain-containing protein, is specifically involved in the cis-splicing of nad5 intron 4 and plays a pivotal role in mitochondrial complex I biogenesis and normal plant growth. © 2021 The Author(s). Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85124634097&doi=10.1093%2fpcp%2fpcab161&partnerID=40&md5=8543bba1cf7b68fe2f2ce5f3edf418be https://scholars.lib.ntu.edu.tw/handle/123456789/626020 |
ISSN: | 00320781 | DOI: | 10.1093/pcp/pcab161 | SDG/關鍵字: | Arabidopsis protein; plant protein; reduced nicotinamide adenine dinucleotide dehydrogenase; Arabidopsis; chloroplast; genetics; intron; metabolism; mitochondrion; RNA splicing; Arabidopsis; Arabidopsis Proteins; Chloroplasts; Introns; Mitochondria; NADH Dehydrogenase; Plant Proteins; RNA Splicing |
顯示於: | 農業化學系 |
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