https://scholars.lib.ntu.edu.tw/handle/123456789/626023
標題: | Cloning and functional characterization of three sesquiterpene synthase genes from Chamaecyparis formosensis Matsumura | 作者: | Hong C.-Y Tsao N.-W Wang S.-Y FANG-HUA CHU |
關鍵字: | Biochemical enzyme characterization; Chamaecyparis formosensis; Gymnosperm; Plant specialized metabolites; Sesquiterpene synthase | 公開日期: | 2022 | 卷: | 321 | 來源出版物: | Plant Science | 摘要: | Terpene synthase (TPS) analysis may contribute to a better understanding of terpenoids biosynthesis and the evolution of phylogenetic taxonomy. Chamaecyparis formosensis Matsumura is an endemic and valuable conifer of Taiwan. Its excellent wood quality, fragrance, and durability make it become the five precious conifers in Taiwan. In this study, three sesquiterpene synthase genes that belong to the TPS-d2 clade were isolated and characterized through in vitro reaction of recombinant protein and in vivo reaction of Escherichia coli heterologous expression system. The main product of Cf-GerA was germacrene A using GC/MS analysis, while the product of Cf-Aco and Cf-Gor were identified as acora-4(14),8-diene and (5R,6R,10S)-α-gorgonene by using NMR analysis. These are the first reported enzymes that biosynthesize acora-4(14),8-diene and (5 R,6 R,10 S)-α-gorgonene. Both sesquiterpene synthases may isomerize the farnesyl pyrophosphate substrate to nerolidyl pyrophosphate for further cyclization. Cf-Aco may catalyze 1,6-cyclization of nerolidyl cation while Cf-Gor may catalyze through an uncharged intermediate, isogermacrene A. © 2022 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85130532657&doi=10.1016%2fj.plantsci.2022.111315&partnerID=40&md5=78a951611edf7852b6eff02f69ca453a https://scholars.lib.ntu.edu.tw/handle/123456789/626023 |
ISSN: | 01689452 | DOI: | 10.1016/j.plantsci.2022.111315 | SDG/關鍵字: | recombinant protein; sesquiterpene; transferase; Chamaecyparis; Escherichia coli; genetics; metabolism; molecular cloning; phylogeny; Alkyl and Aryl Transferases; Chamaecyparis; Cloning, Molecular; Escherichia coli; Phylogeny; Recombinant Proteins; Sesquiterpenes |
顯示於: | 森林環境暨資源學系 |
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