https://scholars.lib.ntu.edu.tw/handle/123456789/626043
標題: | Effect of lactobacillus rhamnosus gg on energy metabolism, leptin resistance, and gut microbiota in mice with diet-induced obesity | 作者: | Cheng Y.-C JE-RUEI LIU |
關鍵字: | Gut microbiota; Lactobacillus rhamnosus; Leptin resistance; Obesity | 公開日期: | 2020 | 卷: | 12 | 期: | 9 | 起(迄)頁: | 1-13 | 來源出版物: | Nutrients | 摘要: | Obesity is closely associated with various metabolic disorders, including leptin resistance, which is characterized by high circulating leptin levels. Probiotics can decrease circulating leptin levels by alteration of the gut microbiota. Thus, they may have anti-obesogenic effects. In this study, the effects of administration of a probiotic bacterium, Lactobacillus rhamnosus GG (LGG), on gut microbiota and modulation of leptin resistance were evaluated in mice. Male Balb/C mice aged 7 weeks were fed either a normal diet (ND), high-fat diet (HFD), HFD supplemented with low-dose LGG (108 CFU/mouse/day), or HFD supplemented with high-dose LGG (1010 CFU/mouse/day) for 10 weeks. Significantly increased body weight, epididymal fat weight, and decreased leptin responsiveness to exogenous leptin treatment and ratio of villus height to crypt depth were observed in the HFD-fed mice compared to the ND-fed mice. Moreover, a remarkable increase in the proportion of Proteobacteria and ratio of Firmicutes/Bacteroidetes in the fecal microbiota were also observed in the HFD-fed mice. Supplementation of HFD with high-dose LGG restored exogenous leptin responsiveness, increased the ratio of villus height to crypt depth, and decreased the proportion of Proteobacteria in fecal microbiota. These findings suggest that LGG supplementation might alleviate leptin resistance caused by an HFD through the improvement of the digestive health of the host. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089992993&doi=10.3390%2fnu12092557&partnerID=40&md5=04aefd4d1ce4a8dfab8a3c9c2d0c6db8 https://scholars.lib.ntu.edu.tw/handle/123456789/626043 |
ISSN: | 20726643 | DOI: | 10.3390/nu12092557 | SDG/關鍵字: | genomic DNA; leptin; RNA 16S; suppressor of cytokine signaling 3; leptin; probiotic agent; animal experiment; animal model; Article; Bacteroidetes; body weight; body weight loss; colony forming unit; controlled study; diet supplementation; energy metabolism; enzyme linked immunosorbent assay; epididymis fat; Firmicutes; histology; intestine flora; Lactobacillus rhamnosus; lipid diet; male; metagenomics; mouse; mRNA expression level; nonhuman; nucleotide sequence; obesity; protein expression; real time polymerase chain reaction; RNA extraction; animal; Bagg albino mouse; blood; disease model; drug effect; energy metabolism; intestine flora; Lactobacillus rhamnosus; metabolism; obesity; Animals; Disease Models, Animal; Energy Metabolism; Gastrointestinal Microbiome; Lactobacillus rhamnosus; Leptin; Male; Mice; Mice, Inbred BALB C; Obesity; Probiotics |
顯示於: | 生物科技研究所 |
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