https://scholars.lib.ntu.edu.tw/handle/123456789/626050
標題: | Engineering plant resistance to tomato yellow leaf curl Thailand virus using a phloem-specific promoter expressing hairpin RNA | 作者: | Tzean Y Chang H.-H Tu T.-C Hou B.-H Chen H.-M Chiu Y.-S Chou W.-Y LI CHANG Yeh H.-H. |
關鍵字: | Constitutive promoter; Phloem-specific promoter; RNA interference; Tomato yellow leaf curl disease; Transgenic plant engineering | 公開日期: | 2020 | 卷: | 33 | 期: | 1 | 起(迄)頁: | 87-97 | 來源出版物: | Molecular Plant-Microbe Interactions | 摘要: | Transgenic approaches employing RNA interference (RNAi) strategies have been successfully applied to generate desired traits in plants; however, variations between RNAi transgenic siblings and the ability to quickly apply RNAi resistance to diverse cultivars remain challenging. In this study, we assessed the promoter activity of a cauliflower mosaic virus 35S promoter (35S) and a phloem-specific promoter derived from rice tungro bacilliform virus (RTBV) and their efficacy to drive RNAi against the endogenous glutamate-1-semialdehyde aminotransferase gene (GSA) that acts as a RNAi marker, through chlorophyll synthesis inhibition, and against tomato yellow leaf curl Thailand virus (TYLCTHV), a begomovirus (family Geminiviridae) reported to be the prevalent cause of tomato yellow leaf curl disease (TYLCD) in Taiwan. Transgenic Nicotiana benthamiana expressing hairpin RNA of GSA driven by either the 35S or RTBV promoter revealed that RTBV |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077666650&doi=10.1094%2fMPMI-06-19-0158-R&partnerID=40&md5=855832bb22e404569abdb0e4ef095c19 https://scholars.lib.ntu.edu.tw/handle/123456789/626050 |
ISSN: | 08940282 | DOI: | 10.1094/MPMI-06-19-0158-R | SDG/關鍵字: | RNA; Begomovirus; disease resistance; genetics; phloem; physiology; promoter region; tomato; transgenic plant; virology; Begomovirus; Disease Resistance; Lycopersicon esculentum; Phloem; Plants, Genetically Modified; Promoter Regions, Genetic; RNA |
顯示於: | 植物病理與微生物學系 |
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