https://scholars.lib.ntu.edu.tw/handle/123456789/626078
標題: | Identification of MaWRKY40 and MaDLO1 as Effective Marker Genes for Tracking the Salicylic Acid-Mediated Immune Response in Bananas | 作者: | Tzean Y Hou B.-H Tsao S.-M Chen H.-M Cheng A.-P Chen E.G Chou W.-Y Chao C.-P WEI-CHIANG SHEN Chen C.-C Lee M.-C Ashraf I Yeh H.-H. |
關鍵字: | Banana; Cucumber mosaic virus; Fusarium oxysporum f. sp. cubense tropical race 4; Marker gene; Plant immune response; Salicylic acid | 公開日期: | 2021 | 卷: | 111 | 期: | 10 | 起(迄)頁: | 1800-1810 | 來源出版物: | Phytopathology | 摘要: | Bananas are among the world’s most important cash and staple crops but are threatened by various devastating pathogens. The phytohormone salicylic acid (SA) plays a key role in the regulation of plant immune response. Tracking the expression of SA-responsive marker genes under pathogen infection is important in pathogenesis elucidation. However, the common SA-responsive marker genes are not consistently induced in different banana cultivars or different organs. Here, we conducted transcriptome analysis for SA response of a banana cultivar, ‘Pei-Chiao’ (Cavendish, AAA genome), and identified three genes, MaWRKY40, MaWRKY70, and Downy Mildew Resistant 6 (DMR6)-Like Oxygenase 1 (MaDLO1) that are robustly induced upon SA treatment in both the leaves and roots. Consistent induction of these three genes by SA treatment was also detected in both the leaves and roots of bananas belonging to different genome types such as ‘Tai-Chiao No. 7’ (Cavendish, AAA genome), ‘Pisang Awak’ (ABB genome), and ‘Lady Finger’ (AA genome). Furthermore, the biotrophic pathogen cucumber mosaic virus elicited the expression of MaWRKY40 and MaDLO1 in infected leaves of susceptible cultivars. The hemibiotrophic fungal pathogen Fusarium oxysporum f. sp. cubense tropical race 4 (TR4) also consistently induced the expression of MaWRKY40 and MaDLO1 in the infected roots of the F. oxysporum f. sp. cubense TR4-resistant cultivar. These results indicate that MaWRKY40 and MaDLO1 can be used as reliable SA-responsive marker genes for the study of plant immunity in banana. Revealing SA-responsive marker genes provides a stepping stone for further studies in banana resistance to pathogens. © 2021 The American Phytopathological Society |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122610768&doi=10.1094%2fPHYTO-01-21-0017-R&partnerID=40&md5=f1224dabef94fc429352cdd501baf42e https://scholars.lib.ntu.edu.tw/handle/123456789/626078 |
ISSN: | 0031949X | DOI: | 10.1094/PHYTO-01-21-0017-R | SDG/關鍵字: | salicylic acid; crop; genetics; immunity; Musa; plant disease; Crops, Agricultural; Immunity; Musa; Plant Diseases; Salicylic Acid |
顯示於: | 植物病理與微生物學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。