The Interference of Medium on Gas Chromatographic Analysis of Diesel in Microbial Degradation and its Elimination [培養基質對微生物降解柴油氣相層析分析之千擾與消除]
Journal
Taiwanese Journal of Agricultural Chemistry and Food Science
Journal Volume
59
Journal Issue
2
Pages
64-69
Date Issued
2021
Author(s)
Abstract
The purpose of this study is to evaluate the strains that have the potential to degrade diesel compounds, such as BS, NO.7 and EMI (the mixtures of bacteria), which have been screened after diesel domestication and degradation tests. For further application of bioenhancement and bioremediation of diesel-contaminated sites, these diesel-degrading strains need to be produced in large scales inexpensively. The use of soybean flour as the culture medium of Bacillus sp. and the addition of excipients during the production of bacterial powder can not only increase the stability of the bacterial powder and its shelf life, but also increase its degradation of diesel compounds after immobilization. The results indicated that the addition of soybean flour and excipients generated interference signals in the GC/FID analysis, which affected the quantification of diesel compounds. In this study, we found that the interference signals mainly come from the organic components in the soy flour, which is the culture medium used for the production of bacterial powder. After the diesel degradation test in water and the long-term degradation experiment in soil, the results of GC/FID analysis showed that the total petroleum hydrocarbon (TPH) signal of diesel with the bacterial powder of Bacillus sp. was significantly reduced. It was confirmed that the soybean flour and excipients contained in the bacterial powder can be utilized by microorganisms. Finally, it has been demonstrated that the use of soybean flour to produce bacterial powder of Bacillus sp. has considerable potential for application in bioremediation of diesel contaminated sites. © 2021 Chinese Agricultural Chemical Society. All rights reserved.
Subjects
Bacillus sp.; Bioremediation; Diesel degradation; GC/FID; Soybean Flour
Type
journal article