The Potential Biocontrol Agent Paenibacillus polymyxa TP3 Produces Fusaricidin-Type Compounds Involved in the Antagonism Against Gray Mold Pathogen Botrytis cinerea
Journal
Phytopathology
Journal Volume
112
Journal Issue
4
Pages
775-783
Date Issued
2022
Author(s)
Abstract
Paenibacillus polymyxa is a beneficial bacterium for plant health. P. polymyxa TP3 exhibits antagonistic activity toward Botrytis cinerea and alleviates gray mold symptoms on the leaves of strawberry plants. Moreover, suppression of gray mold on the flowers and fruits of strawberry plants in field trials, including vegetative cells and endospores, was demonstrated, indicating the potential of strain TP3 as a biological control agent. To examine the anti-B. cinerea compounds produced by P. polymyxa TP3, we performed matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and fusaricidin-corresponding mass spectra were detected. Moreover, fusaricidin-related signals appeared in imaging mass spectrometry of TP3 when confronted with B. cinerea. By using liquid chromatography mass spectrometry-based molecular networking approach, we identified several fusaricidins including a new variant of mass/charge ratio 917.5455 with serine in the first position of the hexapeptide. Via advanced mass spectrometry and network analysis, fusaricidin-type compounds produced by P. polymyxa TP3 were efficiently disclosed and were presumed to play roles in the antagonism against gray mold pathogen B. cinerea. © 2022 The American Phytopathological Society
Subjects
Botrytis cinerea; disease control; fusaricidin-type compounds; Paenibacillus polymyxa; strawberry gray mold
SDGs
Other Subjects
peptide fragment; splenotritin; thymopoietin; Botrytis; Fragaria; genetics; microbiology; Paenibacillus polymyxa; plant disease; prevention and control; Botrytis; Fragaria; Paenibacillus polymyxa; Peptide Fragments; Plant Diseases; Thymopoietins
Type
journal article