https://scholars.lib.ntu.edu.tw/handle/123456789/626220
標題: | Butyrophilin 3A1 binds phosphorylated antigens and stimulates human γδ T cells | 作者: | Vavassori S Kumar A Wan G.S Ramanjaneyulu G.S Cavallari M El Daker S Beddoe T Theodossis A Williams N.K Gostick E Price D.A Soudamini D.U KIEN-VOON KONG Olivo M Rossjohn J Mori L De Libero G. |
公開日期: | 2013 | 卷: | 14 | 期: | 9 | 起(迄)頁: | 908-916 | 來源出版物: | Nature Immunology | 摘要: | Human T cells that express a T cell antigen receptor (TCR) containing γ-chain variable region 9 and δ-chain variable region 2 (V γ 9V δ 2) recognize phosphorylated prenyl metabolites as antigens in the presence of antigen-presenting cells but independently of major histocompatibility complex (MHC), the MHC class I-related molecule MR1 and antigen-presenting CD1 molecules. Here we used genetic approaches to identify the molecule that binds and presents phosphorylated antigens. We found that the butyrophilin BTN3A1 bound phosphorylated antigens with low affinity, at a stoichiometry of 1:1, and stimulated mouse T cells with transgenic expression of a human V γ 9V δ 2 TCR. The structures of the BTN3A1 distal domain in complex with host-or microbe-derived phosphorylated antigens had an immunoglobulin-like fold in which the antigens bound in a shallow pocket. Soluble V γ 9V δ 2 TCR interacted specifically with BTN3A1-antigen complexes. Accordingly, BTN3A1 represents an antigen-presenting molecule required for the activation of V γ 9V δ 2 T cells. © 2013 Nature America, Inc. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84883147590&doi=10.1038%2fni.2665&partnerID=40&md5=6a588c3d057600c08cb99815a743431b https://scholars.lib.ntu.edu.tw/handle/123456789/626220 |
ISSN: | 15292908 | DOI: | 10.1038/ni.2665 | SDG/關鍵字: | butyrophilin 3A1; CD1 antigen; cell protein; major histocompatibility antigen class 1; multiprotein complex; prenylamine; T lymphocyte receptor; unclassified drug; antigen presenting cell; article; binding affinity; chromosome 6; controlled study; gamma delta T lymphocyte; gene expression; genetic identification; human; human cell; major histocompatibility complex; priority journal; protein binding; protein domain; protein folding; protein phosphorylation; protein structure; stoichiometry; T lymphocyte; T lymphocyte activation; transgenics; Animals; Antigen Presentation; Antigen-Presenting Cells; Antigens; Antigens, CD; Chromosomes, Human, Pair 6; Humans; Mice; Mice, Transgenic; Models, Molecular; Organophosphates; Phosphorylation; Protein Binding; Protein Conformation; Receptors, Antigen, T-Cell, gamma-delta; T-Lymphocyte Subsets |
顯示於: | 化學系 |
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