https://scholars.lib.ntu.edu.tw/handle/123456789/626229
標題: | Circulating gut microbiota-related metabolites influence endothelium plaque lesion formation in ApoE knockout rats | 作者: | Chuang H.-L Chiu C.-C Lo C CHENG-CHIH HSU Liu J.-Y Hung S.-W Tsai S.-C Sung H.-H Wang C.-K.L Huang Y.-T. |
公開日期: | 2022 | 卷: | 17 | 期: | 5 | 來源出版物: | PLoS ONE | 摘要: | Atherosclerosis is the main cause of cardiac and peripheral vessel infarction in developed countries. Recent studies have established that gut microbiota and their metabolites play important roles in the development and progression of cardiovascular disease; however, the underlying mechanisms remain unclear. The present study aimed to investigate endothelium plaque lesion formation in ApoE-deficient rats fed a normal chow diet under germ-free (GF) and specific-pathogen-free (SPF) conditions at various time points. There was no difference in serum cholesterol and triglyceride levels between SPF-rats and GF-rats. Histological studies revealed that the GF-rats developed endothelium plaques in the aorta from 26 to 52 weeks, but this was not observed in SPF-rats. GF-rat coronary arteries had moderate-to-severe endothelium lesions during this time period, but SPF-rat coronary arteries had only mild lesion formation. Immunohistochemical staining showed higher accumulation of CD68-positive and arginase-negative foamy-like macrophages on the arterial walls of GF-rats, and expression of TNF-α and IL-6 in foam cells was only observed in GF-rats. In addition, microbial metabolites, including equol derivatives, enterolactone derivatives, indole-3propionate, indole-3-acrylic acid, cholic acid, hippuric acid, and isoquinolone, were significantly higher in the SPF group than in the GF group. In conclusion, our results indicate that gut microbiota may attenuate atherosclerosis development. Copyright: © 2022 Chuang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85129715495&doi=10.1371%2fjournal.pone.0264934&partnerID=40&md5=2a14dcd02361fc52dacca8c7da21cbb2 https://scholars.lib.ntu.edu.tw/handle/123456789/626229 |
ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0264934 | SDG/關鍵字: | CD68 antigen; cholesterol; cholic acid; enterolactone; equol; hippuric acid; indole 3 acrylic acid; indole derivative; indolepropionic acid; interleukin 6; isoquinolone; quinolone derivative; triacylglycerol; tumor necrosis factor; unclassified drug; apolipoprotein E; animal cell; animal experiment; animal model; animal tissue; ApoE gene; artery wall; Article; atherosclerosis; cholesterol blood level; controlled study; disease severity; female; foam cell; gene expression; gene knockout; histology; IL 6 gene; immunohistochemistry; intestine flora; macrophage; nonhuman; pathogenesis; rat; TNF a gene; animal; atherosclerosis; atherosclerotic plaque; endothelium; genetics; Animals; Apolipoproteins E; Atherosclerosis; Endothelium; Gastrointestinal Microbiome; Indoles; Plaque, Atherosclerotic; Rats |
顯示於: | 化學系 |
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